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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Chen-Min Chang Li-Fang Chiou Chun-Che Lin Dar-Bin Shieh Gwo-Bin Lee |
| Copyright Year | 2011 |
| Description | Author affiliation: Institute of Oral Medicine, National Cheng Kung University, Tainan, Taiwan (Li-Fang Chiou; Dar-Bin Shieh) || Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan (Chen-Min Chang; Chun-Che Lin; Gwo-Bin Lee) |
| Abstract | Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach. |
| Starting Page | 193 |
| Ending Page | 196 |
| File Size | 2113914 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424496327 |
| ISSN | 10846999 |
| e-ISBN | 9781424496341 |
| DOI | 10.1109/MEMSYS.2011.5734394 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2011-01-23 |
| Publisher Place | Mexico |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | DNA Microfluidics Magnetic separation Humans Micropumps Muscles Diseases |
| Content Type | Text |
| Resource Type | Article |
| Subject | Condensed Matter Physics Electronic, Optical and Magnetic Materials Mechanical Engineering Electrical and Electronic Engineering |
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