NDLI: Effects of Ketum extract on blowfly Chrysomya megacephala development and detection of mitragynine in larvae sample

Content Provider	IEEE Xplore Digital Library
Author	Rashid, Rumiza Abd Zulkifli, Nurul Farhana Rashid, Reena Abd bt Rosli, Siti Fairuz Sulaiman, Sharifah Hawzah Syed Ahmad, Nazni Wasi
Copyright Year	2012
Description	Author affiliation: Chemistry (Forensic Analysis) Programme, Faculty of Applied Sciences, Universiti Teknologi MARA, (UiTM), 40450 Shah Alam, Selangor, Malaysia (Zulkifli, Nurul Farhana; Rashid, Reena Abd; bt Rosli, Siti Fairuz; Sulaiman, Sharifah Hawzah Syed) \|\| Institute of Forensic Science, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia (Rashid, Rumiza Abd) \|\| Medical Entomology Department, Institute of Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia (Ahmad, Nazni Wasi)
Abstract	Major application of entomology in forensic is in determination of post mortem interval (PMI) based on the age of immature insects that resides the corpse. Chrysomya megacephala is the most forensically significant blowfly in Malaysia. In some death cases due to intoxication, it has been reported that the presence of toxins interrupted the development of immature blowflies, known as larvae that utilizes the corpse's flesh and internal organ and thus disrupt the PMI estimation. Therefore, this study was performed to examine the effects of mitragynine on C.megacephala growth spurt. Mitragynine is an active alkaloid in Mitragyna speciosa Korth or also known as Ketum. This chemical constituent of Ketum accumulated in human liver when consumed. In this study, 110 of C. megacephala larvae were reared on series of cow's liver homogenized with 0, 20g, 40g and 60g of ketum extract. Development rate of C.megacephala was observed by recording larvae length, duration of each development stages and survival rate. Duration of development was recorded in every stages of larvae instar, pupae until the blowfly emerged into adult. Survival rate on the other hand, was recorded based on the number of larvae, pupae and adult that survived to the next cycle of development. All data were compared with control group (0g). Mitragynine from larvae that consumed the mixture were extracted using SPE and detected using HPLC. Calibration curve of mitragynine standard was plotted to obtain the amount of mitragynine concentration in larvae sample. Survival rate and development of larvae exposed to 20g extract were almost similar to control with growth rate delayed by 6 hours and 69 % in survival rate. C.megacephala exposed in 40g extract delayed its growth rate by 12 hours compared to control and only 41% of larvae managed to emerge into adult. The lifecycle of C. megacephala in 60g ketum extract delayed up to 24 hours than the control and has the lowest survival rate among all test groups. From chemical analysis, Mitragynine was detected in all larvae samples exposed to Ketum extact. Blowfly C. megacephala was proven legit for Mitragynine detection in the absence of biological sample on the carrions.
Starting Page	337
Ending Page	341
File Size	285421
Page Count	5
File Format	PDF
ISBN	9781457716324
e-ISBN	9781457716348
DOI	10.1109/ISBEIA.2012.6422898
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-09-23
Publisher Place	Indonesia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Drugs Larvae Ketum Chrysomya megacephala Forensics Humans Liver Mitragynine Post Mortem Interval(PMI) Standards Insects Blowfly Cadaver
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Blowfly, Chrysomya megacephala as an alternative specimen in determination of gunshot residue

Forensic Implications of Blowfly Chrysomya rufifacies (Calliphoridae: Diptera) Development Rates Affected by Ketum Extract

Influence of diclofenac on development rates of forensic blowfly chrysomya megacephala

Importance of entomology in veterinary forensics

DETECTION PURIFICATION AND CHARACTERIZATION OF A POTENT BACTERIOLYTIC FACTOR FROM THE LARVAE OF BLOWFLY CHRYSOMYA MEGACEPHALA

Calliphorids as forensic indicator to facilitate PMI estimation: A case study from Chhattisgarh, India

Analysis of the Transcriptome of Blowfly Chrysomya megacephala (Fabricius) Larvae in Responses to Different Edible Oils

Eicosanoids mediate nodulation reactions to a mollicute bacterium in larvae of the blowfly, Chrysomya megacephala.

Analysis of the Transcriptome of Blowfly Chrysomya megacephala (Fabricius) Larvae in Responses to Different Edible Oils

Effects of Ketum extract on blowfly Chrysomya megacephala development and detection of mitragynine in larvae sample

Similar Documents

Blowfly, Chrysomya megacephala as an alternative specimen in determination of gunshot residue

Forensic Implications of Blowfly Chrysomya rufifacies (Calliphoridae: Diptera) Development Rates Affected by Ketum Extract

Influence of diclofenac on development rates of forensic blowfly chrysomya megacephala

Importance of entomology in veterinary forensics

DETECTION PURIFICATION AND CHARACTERIZATION OF A POTENT BACTERIOLYTIC FACTOR FROM THE LARVAE OF BLOWFLY CHRYSOMYA MEGACEPHALA

Calliphorids as forensic indicator to facilitate PMI estimation: A case study from Chhattisgarh, India

Analysis of the Transcriptome of Blowfly Chrysomya megacephala (Fabricius) Larvae in Responses to Different Edible Oils

Eicosanoids mediate nodulation reactions to a mollicute bacterium in larvae of the blowfly, Chrysomya megacephala.

Analysis of the Transcriptome of Blowfly Chrysomya megacephala (Fabricius) Larvae in Responses to Different Edible Oils

Effects of Ketum extract on blowfly Chrysomya megacephala development and detection of mitragynine in larvae sample