NDLI: Flux Balance Analysis: An Insilico Analysis of Staphylococcus aureus Cell Wall Biosynthesis Pathway Metabolism

Content Provider	IEEE Xplore Digital Library
Author	Johari, S. Dey, P. Sharma, A. Sinha, S. Narain, K. Barua, N.C.
Copyright Year	2013
Description	Author affiliation: North Eastern Inst. of Sci. & Technol., Jorhat, India (Barua, N.C.) \|\| Center for Bioinf. Studies, Dibrugarh Univ., Dibrugarh, India (Dey, P.; Sinha, S.) \|\| Dept. of Chem. & Pharmacy, Univ. Erlangen-Nurnberg, Erlangen, Germany (Sharma, A.) \|\| Centre for Studies in Biotechnol., Dibrugarh Univ., Dibrugarh, India (Johari, S.) \|\| Regional Med. Res. Centre, ICMR, Dibrugarh, India (Narain, K.)
Abstract	Staphylococcus aureus is now being considered under investigation for designing of new inhibitors as the pathogen has acquired resistance against β lactam antibiotics. S. aureus is acquiring resistance against β lactam antibiotics because of penicillin binding proteins (PBPs) coded by mec A gene. These PBPs are considered to be responsible for peptidoglycan cell wall biosynthesis. The peptidoglycan cell wall biosynthesis has therefore been targeted for drug targets identification. Recent researches proved that pathway could be inhibited by drugs like Vancomycin, Daptomycin. Here study has been made to apply insights of Flux Balance analysis(FBA) for peptidoglycan cell wall biosynthesis for identifying antimicrobial drug targets.A comprehensive model of peptidoglycan biosynthesis pathway involves 42 metabolites participating in 13 reactions which are catalysed by 17 proteins. FBA studies has been made for this particular pathway model involving insilico gene deletions and inhibition of PBP2 by analogues of Hamamelitannin (2', 5-di-O-galloyl-D-hamamelose)a plant product from Hamamelis virginiana. The study provided hints about proteins essential for pathway further leading to rational identification of possible drug targets. The analysis showed that apart from PBP2, potential drug targets mtgA, murj(mvin), glycosyltransferase, mrca/B, mrdA, pbpB, p bp4 identified are correlated well with previous researches. This study has helped in rational identification of potential antimicrobial drug targets with application of FBA.
Starting Page	635
Ending Page	640
File Size	768835
Page Count	6
File Format	PDF
ISBN	9780769550138
DOI	10.1109/ICMIRA.2013.132
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-12-21
Publisher Place	India
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Drug PBP2 Inhibitors Antibiotics Biological system modeling FBA Targets Bioinformatics Staphylococcus aureus Immune system
Content Type	Text
Resource Type	Article

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