NDLI: Cell adhesion testing using novel testbeds containing micropatterns of complex nanoengineered multilayer films

Content Provider	IEEE Xplore Digital Library
Author	Shaikh-Mohammed, J. DeCoster, M.A. McShane, J.
Copyright Year	2004
Description	Author affiliation: Inst. of Micromanuf., Louisiana State Univ., Ruston, LA, USA (Shaikh-Mohammed, J.; DeCoster, M.A.; McShane, J.)
Abstract	Methods for producing biomaterial patterns with defined spatial distribution micro- and nano-scale features are important for studying the cellular-level interactions, including basic cell-to-material and cell-to-cell communications. This work reports on the fabrication of substrates to study cell adhesion to multicomponent micropatterns of multilayer films by coupling conventional photolithography and LbL techniques, known as the L-LbL technique. Toward this end, substrates with nanofilm micropatterns of two different bio-functionalities have been fabricated for sPLA/sub 2/ and PLL and were used for in vitro cell-culture studies using neurons, which exhibited preferential and high efficiency and selective adhesion to sPLA/sub 2/ nanofilms. These results support the immediate use of multicomponent micropatterns as biological testbeds for basic studies of cells, and provide a basis for further expansions of the fabrication processes to produce scaffolds for precise definition of cell-to-material and cell-to-cell interactions, such that the resulting constructs mimic in vivo cell organization and behavior.
Starting Page	2671
Ending Page	2674
File Size	978458
Page Count	4
File Format	PDF
ISBN	0780384393
DOI	10.1109/IEMBS.2004.1403767
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-09-01
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Testing Adhesives Nonhomogeneous media Nanobioscience Fabrication Cells (biology) Lithography Phase locked loops In vitro Neurons tissue engineering Microfabrication layer-by-layer (LbL) self-assembly cell culture scaffolds protein patterning
Content Type	Text
Resource Type	Article

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