NDLI: Modeling Study of the Light Stimulation of a Neuron Cell With Channelrhodopsin-2 Mutants

Content Provider	IEEE Xplore Digital Library
Author	Grossman, N. Nikolic, K. Toumazou, C. Degenaar, P.
Copyright Year	1964
Abstract	Channelrhodopsin-2 (ChR2) has become a widely used tool for stimulating neurons with light. Nevertheless, the underlying dynamics of the ChR2-evoked spikes are still not yet fully understood. Here, we develop a model that describes the response of ChR2-expressing neurons to light stimuli and use the model to explore the light-to-spike process. We show that an optimal stimulation yield is achieved when the optical energies are delivered in short pulses. The model allows us to theoretically examine the effects of using various types of ChR2 mutants. We show that while increasing the lifetime and shuttering speed of ChR2 have limited effect, reducing the threshold irradiance by increased conductance will eliminate adaptation and allow constant dynamic range. The model and the conclusion presented in this study can help to interpret experimental results, design illumination protocols, and seek improvement strategies in the nascent optogenetic field.
Sponsorship	IEEE Engineering in Medicine and Biology Society
Page Count	10
File Size	892523
Starting Page	1742
Ending Page	1751
File Format	PDF
ISSN	00189294
Volume Number	58
Issue Number	6
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-06-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Neurons Biomembranes Photoconductivity Lighting Electric potential Adaptation model optogenetics Biophysics channelrhodopsin (ChR2) modeling neurostimulation
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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