NDLI: Assessment of Cytoplasm Conductivity by Nanosecond Pulsed Electric Fields

Content Provider	IEEE Xplore Digital Library
Author	Denzi, A. Merla, C. Palego, C. Paffi, A. Yaqing Ning Multari, C.R. Xuanhong Cheng Apollonio, F. Hwang, J.C.M. Liberti, M.
Copyright Year	1964
Abstract	The aim of this paper is to propose a new method for the better assessment of cytoplasm conductivity, which is critical to the development of electroporation protocols as well as insight into fundamental mechanisms underlying electroporation. For this goal, we propose to use nanosecond electrical pulses to bypass the complication of membrane polarization and a single cell to avoid the complication of the application of the “mixing formulas.” Further, by suspending the cell in a low-conductivity medium, it is possible to force most of the sensing current through the cytoplasm for a more direct assessment of its conductivity. For proof of principle, the proposed technique was successfully demonstrated on a Jurkat cell by comparing the measured and modeled currents. The cytoplasm conductivity was best assessed at 0.32 S/m and it is in line with the literature. The cytoplasm conductivity plays a key role in the understanding of the basis mechanism of the electroporation phenomenon, and in particular, a large error in the cytoplasm conductivity determination could result in a correspondingly large error in predicting electroporation. Methods for a good estimation of such parameter become fundamental.
Sponsorship	IEEE Engineering in Medicine and Biology Society
Starting Page	1595
Ending Page	1603
Page Count	9
File Size	749374
File Format	PDF
ISSN	00189294
Volume Number	62
Issue Number	6
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Conductivity Biomedical measurement Biomembranes Electrodes Current measurement Frequency measurement Time measurement microdosimetry Biological cells biomedical transducers cell cytoplasm conductivity conductivity measurement electroporation
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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