NDLI: Experimental Microdosimetry Techniques for Biological Cells Exposed to Nanosecond Pulsed Electric Fields Using Microfluorimetry

Content Provider	IEEE Xplore Digital Library
Author	Kohler, S. O'Connor, R. P. Vu, T. D. T. Leveque, P. Arnaud-Cormos, D.
Copyright Year	1963
Abstract	In the last decade, high-intensity pulsed electric fields with nanosecond durations (3–300 ns) have found breakthrough biomedical applications, e.g., in cancer treatment and gene therapy; however, the physical mechanisms underlying the interaction between nanosecond pulsed electric fields (nsPEFs) and cells, tissues, or organs are not yet fully elucidated. The precise knowledge of the electromagnetic dose received by the exposed sample at the macroscopic, and better still at the microscopic scale, is essential to complete our understanding of the phenomena involved and for adequate interpretation and reproducibility of the results. In this paper, we report a dosimetric and microdosimetric study of an in vitro exposure setup based on a transverse electromagnetic (TEM) cell that allows the exposure of cells in a Petri dish to nsPEFs. The rectangular and bipolar pulses delivered to the cells had a total duration of 1.2 ns and an amplitude of 2 kV. The electric field in situ was characterized experimentally with a nonmetallic probe and numerically using a finite-difference time-domain algorithm. Results of real-time monitoring of temperature were obtained at the subcellular level by using microfluorimetry, which is a method of imaging temperature by using a fluorescent molecular probe with thermosensitive properties.
Sponsorship	IEEE Microwave Theory and Techniques Society
Starting Page	2015
Ending Page	2022
Page Count	8
File Size	1277918
File Format	PDF
ISSN	00189480
Volume Number	61
Issue Number	5
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-05-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	transverse electromagnetic (TEM) cell Dosimetry finite-difference time-domain (FDTD) method microdosimetry nanosecond pulsed electric field (nsPEF) temperature measurement
Content Type	Text
Resource Type	Article
Subject	Condensed Matter Physics Electrical and Electronic Engineering Radiation

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FDTD-based microdosimetry for high-intensity nanosecond pulsed electric fields (nsPEFs) application

Characterization of a TEM cell-based setup for the exposure of biological cell suspensions to high-intensity nanosecond pulsed electric fields (nsPEFs)

Open Transverse ElectroMagnetic (TEM) cell as applicator of high-intensity nsPEFs and electro-optic measurements

Delivery system setup and characterization for biological cells exposed to nanosecond pulsed electric field

Microchamber Setup Characterization for Nanosecond Pulsed Electric Field Exposure

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Characterization of a 50-Ω Exposure Setup for High-Voltage Nanosecond Pulsed Electric Field Bioexperiments

Specific Absorption Rate Assessment Using Simultaneous Electric Field and Temperature Measurements

Experimental Microdosimetry Techniques for Biological Cells Exposed to Nanosecond Pulsed Electric Fields Using Microfluorimetry

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Open Transverse ElectroMagnetic (TEM) cell as applicator of high-intensity nsPEFs and electro-optic measurements

Delivery system setup and characterization for biological cells exposed to nanosecond pulsed electric field

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