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The evolution of energy-transducing systems. studies with an extremely halophilic archaebacterium (Document No: 19910018410)
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Stan-Lotter, Helga |
| Copyright Year | 1991 |
| Description | The halobacterial ATPase was labeled with C-14-dicyclohexylcarbodiimide and subunit 2 of the enzyme was prepared by electroelution. Subunit 2 was cleaved by several chemical and enzymatic procedures for further preparation of peptides. Immunoreactions (Western blotting) of halobacterial membranes were performed with an antiserum against subunit A of the vacuolar ATPase from Neurospora crassa. A 85 K band (subunit 1) from the membranes of H saccharovorum and from two halobacterial isolates, which were isolated from Permian salt sediments, reacted strongly with the antiserum. The ATPase from the latter isolates resembled the ATPase from H saccharovorum, but had a higher content of acidic amino acids. If it can be verified that the age of the bacterial isolates is in the same range as when deposition of salt occurred, an extremely interesting system for the study of evolutionary questions would be available, since the salt-embedded bacteria presumably did not undergo mutational and selectional events. |
| File Size | 227509 |
| Page Count | 5 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19910018410 |
| Archival Resource Key | ark:/13960/t70w3d00s |
| Language | English |
| Publisher Date | 1991-07-01 |
| Access Restriction | Open |
| Subject Keyword | Membranes Enzyme Activity Peptides Neurospora Bacteria Amino Acids Acidity Antiserums Sediments Deposition Adenosine Triphosphate Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |
