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A comparison of an atpase from the archaebacterium halobacterium saccharovorum with the f1 moiety from the escherichia coli atp synthase
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Hochstein, Lawrence I. Stan-Lotter, Helga |
| Copyright Year | 1989 |
| Description | A purified ATPase associated with membranes from Halobacterium saccharovorum was compared with the F sub 1 moiety from the Escherichia coli ATP Synthase. The halobacterial enzyme was composed of two major (I and II) and two minor subunits (III and IV), whose molecular masses were 87 kDa, 60 kDa, 29 kDa, and 20 kDa, respectively. The isoelectric points of these subunits ranged from 4.1 to 4.8, which in the case of the subunits I and II was consistent with the presence of an excess of acidic amino acids (20 to 22 Mol percent). Peptide mapping of sodium dodecylsulfate-denatured subunits I and II showed no relationship between the primary structures of the individual halobacterial subunits or similarities to the subunits of the F sub 1 ATPase (EC 3.6.1.34) from E. coli. Trypsin inactivation of the halobacterial ATPase was accompanied by the partial degradation of the major subunits. This observation, taken in conjunction with molecular masses of the subunits and the native enzyme, was consistent with the previously proposed stoichiometry of 2:2:1:1. These results suggest that H. saccharovorum, and possibly, Halobacteria in general, possess an ATPase which is unlike the ubiquitous F sub o F sub 1 - ATP Synthase. |
| File Size | 4723661 |
| Page Count | 19 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19890012957 |
| Archival Resource Key | ark:/13960/t18m26s1s |
| Language | English |
| Publisher Date | 1989-03-01 |
| Access Restriction | Open |
| Subject Keyword | Space Biology Stoichiometry Enzymes Escherichia Purification Peptides Molecular Structure Archaebacteria Amino Acids Molecular Weight Biopolymer Denaturation Trypsin Electrophoresis Chemical Composition Chemical Properties Adenosine Triphosphate Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |
