NDLI: Effects of simulated weightlessness on meiosis. fertilization, and early development in mice

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Effects of simulated weightlessness on meiosis. fertilization, and early development in mice

Content Provider	NASA Technical Reports Server (NTRS)
Author	Wolgemuth, D. J.
Copyright Year	1986
Description	The initial goal was to construct a clinostat which could support mammalian cell culture. The clinostat was selected as a means by which to simulate microgravity conditions within the laboratory, by constant re-orientation of cells with respect to the gravity vector. The effects of this simulated microgravity on in-vitro meiotic maturation of oocytes, using mouse as the model system, was investigated. The effects of clinostat rotation on fertilization in-vitro was then examined. Specific endpoints included examining the timely appearance of male and female pronuclei (indicating fertilization) and the efficiency of extrusion of the second polar body. Particular attention was paid to detecting anomalies of fertilization, including parthenogenetic activation and multiple pronuclei. Finally, for the preliminary studies on mouse embryogenesis, a key feature of the clinostat was modified, that of the position of the cells during rotation. A means was found to immobilize the cells during the clinostat reotation, permitting the cells to remain at the axis of rotation yet not interfering with cellular development.
File Size	9280954
Page Count	18
File Format	PDF
Alternate Webpage(s)	http://archive.org/details/NASA_NTRS_Archive_19860020870
Archival Resource Key	ark:/13960/t4pk58m3p
Language	English
Publisher Date	1986-01-01
Access Restriction	Open
Subject Keyword	Space Biology Fertilization Cell Division Culture Techniques Weightlessness Embryos Equipment Specifications Cells Biology Gravitational Effects Space Commercialization Morphology Mice Microgravity Reproduction Biology Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science
Content Type	Text
Resource Type	Technical Report

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