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Ultrasensitive bioluminescent determinations of adenosine triphosphate (atp) for investigating the energetics of host-grown microbes
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Hanks, J. H. Dhople, A. M. |
| Copyright Year | 1975 |
| Description | Stability and optimal concentrations of reagents were studied in bioluminescence assay of ATP levels. Luciferase enzyme was prepared and purified using Sephadex G-100. Interdependencies between enzyme and luciferin concentrations in presence of optimal Mg are illustrated. Optimal ionic strength was confirmed to be 0.05 M for the four buffers tested. Adapted features of the R- and H-systems are summarized, as well as the percentages of ATP pools released from representative microbes by heat and chloroform. |
| File Size | 275990 |
| Page Count | 9 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19760008457 |
| Archival Resource Key | ark:/13960/t1ng9f08f |
| Language | English |
| Publisher Date | 1975-01-01 |
| Access Restriction | Open |
| Subject Keyword | Earth Resources And Remote Sensing Enzymes Microorganisms Bioluminescence Energy Levels Bioassay Insects Chloroform Magnesium Adenosine Triphosphate Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
