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A rapid method for the determination of microbial susceptibility using the firefly luciferase assay for adenosine triphosphate (atp)
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Chappelle, E. W. Tuttle, S. A. Barza, M. Weinstein, L. Picciolo, G. L. Vellend, H. |
| Copyright Year | 1975 |
| Description | Luciferase assay for adenosine triphosphate (ATP) was optimized for pure bacteria in broth in order to evaluate if changes in bacterial ATP content could be used as a rapid measure of antibiotic effect on microorganisms. Broth cultures of log phase bacteria were incubated at 310 K (37 C) for 2.5 hours at antimicrobial concentrations which resulted in the best discrimination between sensitive and resistant strains. Eighty-seven strains of 11 bacterial species were studied for their susceptibility to 12 commonly used antimicrobial agents: ampicillin, Penicillin G, nafcillin, carbenicillin, cephalothin, tetracycline, erythromycin, clindamycin, gentamicin, nitrofurantoin, colistin, and chloramplenicol. The major advantage of the ATP system over existing methods of rapid microbial susceptibility testing is that the assay can be made specific for bacterial ATP. |
| File Size | 59834 |
| Page Count | 2 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19760008454 |
| Archival Resource Key | ark:/13960/t83j87t1m |
| Language | English |
| Publisher Date | 1975-01-01 |
| Access Restriction | Open |
| Subject Keyword | Earth Resources And Remote Sensing Enzymes Microorganisms Bioluminescence Bioassay Antibiotics Insects Bacteria Adenosine Triphosphate Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
