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The effect of growth phase and medium on the use of the firefly adenosine triphosphate (atp) assay for the quantitation of bacteria
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Bush, V. N. Chappelle, E. W. Picciolo, G. L. |
| Copyright Year | 1975 |
| Description | Luciferase assay for adenosine triphosphate (ATP) was used as a rapid method to determine the number of bacteria in a urine sample after nonbacterial components were removed. Accurate cellular ATP values, determined when bacteria were grown in an environment similar to that in which they were found, were necessary for the calculation of bacterial titer in urine. Cellular ATP values vary depending on the extraction method, the cell growth phase, and cell growth conditions. ATP per cell values of stationary E. coli grown in urine were two times greater than ATP per cell values of cells grown in trypticase soy broth. Glucose and urea were examined as possible components responsible for the cellular ATP variation. |
| File Size | 235804 |
| Page Count | 7 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19760008453 |
| Archival Resource Key | ark:/13960/t0ps2k073 |
| Language | English |
| Publisher Date | 1975-01-01 |
| Access Restriction | Open |
| Subject Keyword | Earth Resources And Remote Sensing Urine Enzymes Escherichia Bioluminescence Bioassay Insects Bacteria Ureas Glucose Adenosine Triphosphate Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
