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| Content Provider | frontiers |
|---|---|
| Author | Di Rosa, Francesca Cossarizza, Andrea Hayday, Adrian C. |
| Abstract | Quantitation of Ki-67, a nuclear protein associated with cell cycle, is currently among the topranked methods to evaluate T cell proliferation, especially in human samples ex vivo. Readily detectable levels of Ki-67 mRNA and protein are present during the four cell cycle phases (i.e. G1, S, G2, M) and are down-regulated when cells exit cell cycle and enter into quiescence (i.e. the G0 phase) (1). Originally named after the first monoclonal antibody (mAb) used to identify it (2), Ki-67 protein can now be stained with a series of mAbs with different sensitivities and epitope-specificities, including some mAbs that can detect extremely low levels of the protein, even in quiescent cells (3)(4)(5)(6). From a functional standpoint, Ki-67 supports chromosome architecture organization and nucleolar assembly upon mitosis (7; 8); helps remove cytoplasm from the reassembling nucleus during mitotic exit (9); and regulates heterochromatin compaction and gene expression in proliferating cells (10). This notwithstanding, mutant mice with disrupted Ki-67 expression are vital and fertile, grow normally, and do not show abnormalities in highly proliferative tissues, such as the intestinal epithelium (10).Given these considerations, it is evident that the very frequent use of Ki-67 as a proliferation marker is mistaken: rather, Ki-67 discriminates between cells having detectable Ki-67 expression (Ki-67 + ) in any phase of cell cycle (i.e. G1, S, G2, M), and cells lacking it (Ki-67 -) in the ... |
| ISSN | 16643224 |
| DOI | 10.3389/fimmu.2021.756641 |
| Volume Number | 12 |
| Journal | Frontiers in Immunology |
| Language | English |
| Publisher Date | 2021-09-28 |
| Access Restriction | Open |
| Subject Keyword | Cell Cycle Flow Cytometry T cells Ki-67 DNA dye |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
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