Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | frontiers |
|---|---|
| Author | Metsiou, Despoina Nektaria Deligianni, Despina Giannopoulou, Efstathia Kalofonos, Haralabos Koutras, Angelos Athanassiou, George |
| Abstract | The onset and the progression of cancer, is strongly associated with the dissipation of adhesion forces between cancer cells, thus facilitating the incessant attachment and detachment from the extracellular matrix (ECM) in order to move to the direction of metastasis. During this process, cancer cells undergo mechanical stresses and respond to these stresses with membrane deformation while induce protrusions to invade the surrounding tissues. The cellular response to mechanical forces is inherently related to the reorganization of cytoskeleton, the dissipation of cell-cell junctions and the adhesion to surrounding ECM. Moreover, the role of focal adhesion proteins and particularly the role of vinculin in cell attachment and detachment during migration is critical, indicating the tight cell-ECM junctions which favor or inhibit the metastatic cascade. The biomechanical analysis of these sequences of events may elucidate the tumor progression and the potential of cancer cells for migration and metastasis. In this work we focused on the evaluation of the spreading rate and the estimation of the adhesion strength between breast cancer cells-ECM prior to and post treatment with anti-tumor agents. Specifically, different tamoxifen concentrations, were used for ER+ breast cancer cells whilst even concentrations of Trastuzumab and Pertuzumab for HER2+ respectively. Analysis of cell stiffness indicated an increased elastic Young’s modulus post treatment in both MCF-7 and SKBR-3 cells. The results showed that the post treatment spreading rate was significantly decreased in both types of breast cancer suggesting a lower metastatic potential. Additionally, treated cells required greater adhesion forces to detach from ECM, thus preventing detachment events of cancer cells from ECM, and therefore, the probability for cell motility, migration and metastasis is confined. Furthermore, post detachment and post treatment vinculin levels were increased, indicating tighter cell-ECM junctions, hence limiting the probability for cell detachment and, therefore, cell motility and migration. |
| ISSN | 2234943X |
| DOI | 10.3389/fonc.2022.811508 |
| Volume Number | 12 |
| Journal | Frontiers in Oncology |
| Language | English |
| Publisher Date | 2022-08-16 |
| Access Restriction | Open |
| Subject Keyword | Anti-tumor agents Adhesion forces Vinculin Elasticity Cancer cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|