NDLI: R7 photoreceptor axon targeting depends on the relative levels of lost and found expression in R7 and its synaptic partners

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Jessica Douthit Ariel Hairston Gina Lee Carolyn A. Morrison Isabel Holguera Jessica E. Treisman
Abstract	As neural circuits form, growing processes select the correct synaptic partners through interactions between cell surface proteins. The presence of such proteins on two neuronal processes may lead to either adhesion or repulsion; however, the consequences of mismatched expression have rarely been explored. Here, we show that the Drosophila CUB-LDL protein Lost and found (Loaf) is required in the UV-sensitive R7 photoreceptor for normal axon targeting only when Loaf is also present in its synaptic partners. Although targeting occurs normally in loaf mutant animals, removing loaf from photoreceptors or expressing it in their postsynaptic neurons Tm5a/b or Dm9 in a loaf mutant causes mistargeting of R7 axons. Loaf localizes primarily to intracellular vesicles including endosomes. We propose that Loaf regulates the trafficking or function of one or more cell surface proteins, and an excess of these proteins on the synaptic partners of R7 prevents the formation of stable connections.
e-ISSN	2050084X
DOI	10.7554/eLife.65895
Journal	eLife
Volume Number	10
Language	English
Publisher	eLife Sciences Publications Ltd
Publisher Date	2021-01-01
Publisher Place	United Kingdom
Access Restriction	Open
Subject Keyword	Medicine Science Biology (general) Photoreceptor Synapse Endosome Axon Targeting Matching
Content Type	Text
Resource Type	Article

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R7 photoreceptor axon targeting depends on the relative levels of lost and found expression in R7 and its synaptic partners