NDLI: Deletion of the Response Regulator PhoP Accelerates the Formation of Aerial Mycelium and Spores in Actinosynnema pretiosum

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Peipei Zhang Kunyu Zhang Yayu Liu Jiafang Fu Gongli Zong Xin Ma Guangxiang Cao
Abstract	PhoPR is an important two-component signal transduction system (TCS) for microorganisms to sense and respond to phosphate limitation. Although the response regulator PhoP controls morphological development and secondary metabolism in various Streptomyces species, the function of PhoP in Actinosynnema pretiosum remains unclear. In this study, we showed that PhoP significantly represses the morphological development of the A. pretiosum X47 strain. Production of aerial mycelium and spore formation occurred much earlier in the ΔphoP strain than in X47 during growth on ISP2 medium. Transcription analysis indicated that 222 genes were differentially expressed in ∆phoP compared to strain X47. Chemotaxis genes (cheA, cheW, cheX, and cheY); flagellum biosynthesis and motility genes (flgBCDGKLN, flaD, fliD-R, motA, and swrD); and differentiation genes (whiB and ssgB) were significantly upregulated in ∆phoP. Gel-shift analysis indicated that PhoP binds to the promoters of flgB, flaD, and ssgB genes, and PHO box-like motif with the 8-bp conserved sequence GTTCACGC was identified. The transcription of phoP/phoR of X47 strain was induced at low phosphate concentration. Our results demonstrate that PhoP is a negative regulator that controls the morphological development of A. pretiosum X47 by repressing the transcription of differentiation genes.
e-ISSN	1664302X
DOI	10.3389/fmicb.2022.845620
Journal	Frontiers in Microbiology
Volume Number	13
Language	English
Publisher	Frontiers Media S.A.
Publisher Date	2022-04-01
Publisher Place	Switzerland
Access Restriction	Open
Subject Keyword	Microbiology Actinosynnema Pretiosum Morphological Development Phop Pho Box Tcs
Content Type	Text
Resource Type	Article

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