NDLI: Corticohippocampal circuit dysfunction in a mouse model of Dravet syndrome

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Joanna Mattis Ala Somarowthu Kevin M. Goff Evan Jiang Jina Yom Nathaniel Sotuyo Laura M. Mcgarry Huijie Feng Keisuke Kaneko Ethan M. Goldberg
Abstract	Dravet syndrome (DS) is a neurodevelopmental disorder due to pathogenic variants in SCN1A encoding the Nav1.1 sodium channel subunit, characterized by treatment-resistant epilepsy, temperature-sensitive seizures, developmental delay/intellectual disability with features of autism spectrum disorder, and increased risk of sudden death. Convergent data suggest hippocampal dentate gyrus (DG) pathology in DS (Scn1a+/-) mice. We performed two-photon calcium imaging in brain slice to uncover a profound dysfunction of filtering of perforant path input by DG in young adult Scn1a+/- mice. This was not due to dysfunction of DG parvalbumin inhibitory interneurons (PV-INs), which were only mildly impaired at this timepoint; however, we identified enhanced excitatory input to granule cells, suggesting that circuit dysfunction is due to excessive excitation rather than impaired inhibition. We confirmed that both optogenetic stimulation of entorhinal cortex and selective chemogenetic inhibition of DG PV-INs lowered seizure threshold in vivo in young adult Scn1a+/- mice. Optogenetic activation of PV-INs, on the other hand, normalized evoked responses in granule cells in vitro. These results establish the corticohippocampal circuit as a key locus of pathology in Scn1a+/- mice and suggest that PV-INs retain powerful inhibitory function and may be harnessed as a potential therapeutic approach toward seizure modulation.
e-ISSN	2050084X
DOI	10.7554/eLife.69293
Journal	eLife
Volume Number	11
Language	English
Publisher	eLife Sciences Publications Ltd
Publisher Date	2022-01-01
Publisher Place	United Kingdom
Access Restriction	Open
Subject Keyword	Medicine Science Biology (general) Dravet Syndrome Scn1a Nav1.1 Gabaergic Interneurons Dentate Gyrus
Content Type	Text
Resource Type	Article

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