NDLI: Development of Stretch Stimulation Device for Three-Dimensional Culture of PC12 Cells

Please wait, while we are loading the content...

ANES/ASME Joint National Solar Energy Week

ASME Citrus Engineering Symposium

ASME India Oil and Gas Pipeline Conference

ASME International Mechanical Engineering Congress and Exposition

ASME 2002 International Mechanical Engineering Congress and Exposition(IMECE2002)

ASME 2003 International Mechanical Engineering Congress and Exposition(IMECE2003)

ASME 2004 International Mechanical Engineering Congress and Exposition(IMECE2004)

ASME 2005 International Mechanical Engineering Congress and Exposition(IMECE2005)

ASME 2006 International Mechanical Engineering Congress and Exposition(IMECE2006)

ASME 2007 International Mechanical Engineering Congress and Exposition(IMECE2007)

ASME 2008 International Mechanical Engineering Congress and Exposition(IMECE2008)

ASME 2009 International Mechanical Engineering Congress and Exposition(IMECE2009)

ASME 2010 International Mechanical Engineering Congress and Exposition(IMECE2010)

ASME 2011 International Mechanical Engineering Congress and Exposition(IMECE2011)

ASME 2012 International Mechanical Engineering Congress and Exposition(IMECE2012)

ASME 2013 International Mechanical Engineering Congress and Exposition(IMECE2013)

ASME 2014 International Mechanical Engineering Congress and Exposition(IMECE2014)

ASME 2015 International Mechanical Engineering Congress and Exposition(IMECE2015)

ASME 2016 International Mechanical Engineering Congress and Exposition(IMECE2016)

ASME 2017 International Mechanical Engineering Congress and Exposition(IMECE2017)

ASME 2018 International Mechanical Engineering Congress and Exposition(IMECE2018)

Volume 7: Fluids Engineering

Volume 13: Design, Reliability, Safety, and Risk

Volume 4A: Dynamics, Vibration, and Control

Volume 6A: Energy

Volume 1: Advances in Aerospace Technology

Volume 2: Advanced Manufacturing

Volume 5: Engineering Education

Volume 4B: Dynamics, Vibration, and Control

Volume 12: Materials: Genetics to Structures

Volume 10: Micro- and Nano-Systems Engineering and Packaging

Volume 9: Mechanics of Solids, Structures, and Fluids

Volume 11: Acoustics, Vibration, and Phononics

Volume 8A: Heat Transfer and Thermal Engineering

Volume 3: Biomedical and Biotechnology Engineering

Development of Chemotherapy System Using Plasma Activated Medium to Enhance PC12 Axonal Extension

Development of Three-Dimensional DC Electric Field Stimulation Bio-Reactor for Axonal Outgrowth Enhancement

Development of Stretch Stimulation Device for Three-Dimensional Culture of PC12 Cells

Design and Fabrication of Pneumatic Soft Gripper

Cellular Automaton and Finite Element Hybrid Simulation to Predict Axonal Extension Enhancement of Nerve Cell Under Mechanical Stimulation

Reproduction of Kinematic Behavior of Elastic Lamellae in the Thoracic Aortic Media

Mechanical Properties of the Human Elbow Bones Measured by Nanoindentation and Microindentation

Mechanical Property Determination of a Stereolithographic Resin Subjected to Compressive Loading

Biomaterial Models Adjustment and Comparison for Ultra-High Molecular Weight Polyethylene in Finite Element Models

On Continuum Based Multiscale Modelling of Engineered Soft Tissue Constructs

Design and Validation of a Low-Cost Non-Invasive Device to Detect Overnight Hypoglycemia

Pre-Surgical Planning of Screw-Position Arrangement for the Femur Fractures With a Custom APP

Design and Validation of a Low-Cost Heart Health Monitoring Device

Biomedical Devices Using Shape Memory Polymer Foams for Treatment of Intracranial Aneurysms

Modified Laparoscopic Tool for Enhanced Haptic Feedback

Minimizing Pain in Below Knee Amputees’ Patients Wearing Prosthetic Socket by Increasing Flexibility in Specific Relief Areas

An Approach to Capture Humidity From Exhaled Air

Development of a Passive Prosthetic Ankle With Slope Adapting Capabilities

Design of a Smart Glove Using Flexible Technology for Artificial Gripper

A Small Radio Frequency Sensor for Microwave Tumor Ablation

Smartphone-Based Device for Monitoring Chemical Pollutants in Water

Canal Design for SLA Printing of Closed Geometries

A Numerical Study of the Influence of Different Factors on Mechanical Characterization of Tumors via a 2D Tactile Sensor

Design and Prototype Development of a Reconfigurable Wheelchair With Stand-Sit-Sleep Configurations

Demonstration and Experimental Validation of Plastic-Encased Resonant Ultrasonic Piezoelectric Actuator for MRI-Guided Surgical Robots

Continuous Cell Sorting by Dielectrophoresis in a Straight Microfluidic Channel

Neural Network Modeling of Maximum Insertion Force of Bevel-Tip Surgical Needle

Relating Bone Intra-Cortical Elastic Stiffness to EDX Spectroscopy Mineralization Measurements

Deep Convolutional Neural Networks for Breast Image Analysis on Holographic Microwave Imaging

A Modular Test Platform for Micromechanical Tensile Testing of Soft Biomaterials

Compliance Effect on the Flow Condition in Vascular In Vitro Experiments

Investigating the Progression of Alzheimer’s Disease Using Digital Volume Correlation Algorithm and Strain As a Metric

Efficient Uncertainty Quantification for Biotransport in Tumors With Uncertain Material Properties

Numerical Investigation of Heat Transfer in Tissues During Therapeutic Hyperthermia

Influence of Non-Newtonian Rheology on Mass Transfer From a Biofluid in Separated and Reattached Flows

Electrokinetic Remediation of Contaminated Soils With Chromium

Coupling Immunofluorescence and Optoelectrokinetic Technique for Escherichia Coli Detection and Quantification in Water

Exploring Training Methodologies Towards the Improvement of Elderly Balance

Investigation of Forces in Deep Hole Bone Drilling

A New Wideband Microwave Antenna for Breast Cancer Detection

A Clinical Experiment on Infant Applied Pressures During Breastfeeding

Viscoelastic Properties of the L3-L4 Myofascial Tissue in Ankylosing Spondylitis Patients

Three-Dimensional Cephalometric Analysis Using Computed Tomographic Imaging

Deposition Control of a FDM 3D Printer Based Direct Writing System for Hydrogel Molding in Microfluidic Devices Fabrication

Experimental Study of Bio-Polymer Knee Implant

Hydrodynamic Recruitment of Leukocytes Is Influenced by Adherent Cell Morphology

Monte Carlo Simulation of the Laser-Induced Temperature Dynamics in Very Thin Scattering and Absorbing Biological Layer Piles

The Wisdom of Crowds Approach to Influenza-Rate Forecasting

Non-Linear Analysis of Bio-Structures Through Refined Beam Models

Patellofemoral Pain Syndrome: Sensitivity Analysis of Muscle Parameters for Expedited Recovery Utilizing an OpenSim Model for Lower Extremities

Evaluation of Soft Material Fracture Behavior via Indentation Testing With a Needle-Like Indenter

Reconstruction of Swirling Blood Flow in the Heart and Aorta on the Basis of Measurements of Dynamic Geometry and Elastic Properties of the Flow Channel

Computational Modeling of Coagulopathy for Decision Support

Fluid-Structure Interaction of Blood Flow in Human Aorta Under Dynamic Conditions: A Numerical Approach

Validation of a Finite Element Model of the Mechanical Performance of Surgical Knots of Varying Topology

A Stochastic Finite Element Method for Simulating Trabecular Bone

Modeling Heat Regulation With a Structured Mesh, Finite Volume Approach in a Voxelized Domain

The Influence of Primary Blast Wave on the Posterior Part of the Eyeball

Degradation Modeling of Bioabsorbable Polymer Stent

A Computational Model of Continuous Hollow Cerebrovascular Arterioles Using a Fractal L-System

A Biomechanical and Thermal Analysis for Bone Augmentation of the Proximal Femur

Comparison of Ablation Volume Produced With Multi-Tine Dry Type and Wet Type Electrodes During Radio Frequency Ablation: An In Vitro Study

Computational Analysis for Validation of Blast Induced Traumatic Brain Injury and Protection of Combat Helmet

Ranking of Biomechanical Metrics to Describe Human Response to Impact-Induced Damage

Computational Modeling of Blunt Impact to Head and Correlation of Biomechanical Measures With Medical Images

Indent Depth in the Clay Backing for Ceramic Armor

The Strain Rates of the Brain and Skull Under Dynamic Loading

Computational Human Torso Model Validation for Frontal Blunt Trauma

Brain Tissue Material and Damage Properties for Blast Trauma

Pelvic Injury Survival Analysis for a Finite Element Human Body Model Using Multiple Data Sets

Develop a Flexible Regenerative Exoskeleton to Assist Walking

Arterial Wall Motion and its Dynamic Modeling for Arterial Stiffness and Damping

Material and Posture Modeling for Sleeping on Soft Low-Density Porous Material

Virtual Interaction Between Patients and Occupational Therapists Using an Assistive Robotic Device With Cyber-Physical System

Estimation of Hip and Ankle Visco-Elastic Parameters During Quiet Standing

Is Linear Camera Space Manipulation Impervious to Systematic Distortions?

Hypo/Hyperglycemic Constrained Design of IV Insulin Control for Type 1 Diabetic Patients With Meal and Initial Condition Uncertainties Using Sequential Quadratic Programming

Chance Constraint Based Design of IV Insulin Control for Type 1 Diabetic Patients Under Model and Meal Uncertainties

Viscoelastic Characterization of Woven Dacron for Aortic Grafts by Using Direction-Dependent Quasi-Linear Viscoelasticity

2-D Inverse Dynamics Knee Model: Aligning Anatomical Knee Model With Squatting Kinematic Data Using Ligament Forces

A Dynamic Escape Problem of Molecular Motors

Development of a Low-Cost Mechanical Model of a Human Head

Finite Element Analysis of Dynamics of Human Muscle Compressed by Fabric Sleeve

Gait Analysis for Muscular Forces Evaluation in Human Movement: Integration Protocol of Typical Measurement Methods

Study of Head Concussions on Female Soccer Players

Neck Loading Model of a Child in a Car Seat

Estimation Method of 3D Position of Guidewire in Endovascular Treatment

Computational Study of Onset Dynamics in Neuron-Spiking With Threshold Adaptation

Reproduction Analysis of Injury Condition Using Finite Element Modeling of the Head in Cases With Traumatic Higher Brain Dysfunction Caused by Traffic Accidents

Developing Activated Carbon From Date Seeds and Carbon Fiber for Use in Liver Support Devices

Shear Stress Induced Calcium Dependent Nuclear Deformation in Epithelial Cells

Acoustic Radiation of Axially Stepped-Thickness Piezoelectric Cylindrical Shells

Investigation of the Scattering of Focused Ultrasonic Waves at Bones

Influence of Brain Cooling on Frequency Characteristics of the Epileptic Focus and its Surrounding Area

Fluid Impact Under Various Tapping Conditions for Biomedical Application (Shirodhara)

A Study of a Mechanism of Cell Proliferation Promotion of Cultured Osteoblasts by Mechanical Vibration

Determination of Mechanical Properties of Human Skull With Modal Analysis

Experimental Identification of Model Parameters and the Statistical Processing Using a Nonlinear Oscillator Applied to EEG Analysis

Volume 6B: Energy

Volume 8B: Heat Transfer and Thermal Engineering

ASME International Pipeline Geotechnical Conference

ASME Power Conference

ASME Turbine Blade Tip Symposium

ASME/IEEE Joint Rail Conference

ASME/JSME Thermal Engineering Joint Conference

ASME/NRC Pump and Valve Symposium

ASME/USCG Workshop on Marine Technology and Standards

CANEUS: MNT for Aerospace Applications

Dynamic Systems and Control Conference

Energy Nanotechnology International Conference

Energy Sustainability

Engineering Systems Design and Analysis

Engineering Technology Conference on Energy

Fluid Power Systems Technology

Fluids Engineering Division Summer Meeting

Frontiers in Biomedical Devices

Gas Turbine India Conference

Global Congress on NanoEngineering for Medicine and Biology

Heat Transfer Summer Conference

High Temperature Reactor Technology

Information Storage and Processing Systems

Integrated Nanosystems: Design, Synthesis, and Applications

Internal Combustion Engine Division Fall Technical Conference

Internal Combustion Engine Division Spring Technical Conference

International Conference on Fluidized Bed Combustion

International Conference on Fuel Cell Science, Engineering and Technology

International Conference on Integration and Commercialization of Micro and Nanosystems

International Conference on Micro/Nanoscale Heat Transfer

International Conference on Nanochannels, Microchannels, and Minichannels

International Conference on Nuclear Engineering

International Conference on Offshore Mechanics and Arctic Engineering

International Conference on Radioactive Waste Management and Environmental Remediation

International Design Engineering Technical Conferences and Computers and Information in Engineering Conference

International Electronic Packaging Technical Conference and Exhibition

International Heat Transfer Conference

International Joint Power Generation Conference

International Joint Tribology Conference

International Manufacturing Science and Engineering Conference

International Pipeline Conference

International Solar Energy Conference

International Symposium on Flexible Automation

Multifunctional Nanocomposites and Nanomaterials International Conference

Noise Control and Acoustics Division Conference

North American Waste-to-Energy Conference

Pressure Technology

Pressure Vessels and Piping Conference

Rail Transportation Division Conference

Small Modular Reactors Symposium

Smart Materials, Adaptive Structures and Intelligent Systems

Summer Bioengineering Conference

Turbo Expo: Power for Land, Sea, and Air

Verification and Validation

Water Quality, Drought, Human Health and Engineering Conference

Wind Energy Symposium

World Conference on Innovative Virtual Reality

World Tribology Congress

Development of Stretch Stimulation Device for Three-Dimensional Culture of PC12 Cells

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Imura, Madoka Sakiyama, Ryota Yamamoto, Koji Morita, Yusuke Nakamachi, Eiji
Copyright Year	2018
Abstract	Enhancement of nerve axonal extension by using the extracellular environmental stimulation were reported. In this study, we focused on the stretch stimulation, and developed a 3D cell culture system to mimic the in vivo extracellular matrices and investigated the fundamental mechanism of axonal extension enhancement. Firstly, we fabricated the stretch stimulation device. The rat phenocromocytoma cells (PC12), the nerve-like cells, embedded in the collagen gel were poured into the stretch chamber. It was set in the stretch stimulation device, which could load the strain to the collagen gel. Secondly, we determined the structure of the stretch chamber to implement the uniform strain distribution in the culture region. Using the finite element (FE) analyses, we confirmed that the uniform strain is assigned in a region of 2.7 × 3.0 × 0.5 mm in the culture region, which is the candidate for the observation region. Thirdly, PC12 cells axonal extension under uniaxial cyclic stretch stimulation (4% strain, 1 Hz) of 24 hours was carried out. After 96 hours’ culture, we observed the 3D morphology of PC12 cells by the multiphoton excitation fluorescence microscope (MPM). Finally, we confirmed the availability of our stretch stimulation device and the enhancement effect of axonal extension.
File Format	PDF
ISBN	9780791852026
DOI	10.1115/IMECE2018-86643
Volume Number	Volume 3: Biomedical and Biotechnology Engineering
Conference Proceedings	ASME 2018 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2018-11-09
Publisher Place	Pittsburgh, Pennsylvania, USA
Access Restriction	Subscribed
Subject Keyword	Fluorescence Excitation Finite element analysis Microscopes Stress
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in