NDLI: Development of Three-Dimensional DC Electric Field Stimulation Bio-Reactor for Axonal Outgrowth Enhancement

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Development of Three-Dimensional DC Electric Field Stimulation Bio-Reactor for Axonal Outgrowth Enhancement

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Tanaka, Shohei Sakiyama, Ryota Yamamoto, Koji Morita, Yusuke Nakamachi, Eiji
Copyright Year	2018
Abstract	Numerous studies of electrical stimulation effects on the nerve regeneration have been carried out. However, there were very few investigations which adopt the 3D culture that mimics the in vivo environment. In this study, we designed and fabricated a new 3D direct current electric field (DCEF) stimulation bio-reactor and investigated the effectiveness on the axonal outgrowth enhancement. We searched an optimum structure using the finite element (FE) analyses to obtain a uniform DCEF in the culture region. A measurement result of DCEF strength showed an agreement with FE results. The rat phenocromocytoma cells (PC12) were disseminated in the collagen gel and 3D culture was performed. We observed the morphologies of cell bodies and neurites using the multiphoton excitation fluorescence microscope (MPM). Both increases in 11.3% of mean axonal length and in 4.2% of axogenesis rate, under the condition of 5.0 mV/mm on 6 hours a day for 4 days, were obtained. Further, there was a tendency of longer connecting distance between cell bodies in the DCEF group than one in the Control group. As a result, we validated the efficacies of our stimulation, both for the axonal extension and the neural network generation, using our newly developed bio-reactor.
File Format	PDF
ISBN	9780791852026
DOI	10.1115/IMECE2018-86637
Volume Number	Volume 3: Biomedical and Biotechnology Engineering
Conference Proceedings	ASME 2018 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2018-11-09
Publisher Place	Pittsburgh, Pennsylvania, USA
Access Restriction	Subscribed
Subject Keyword	Structural optimization Bioreactors Artificial neural networks Fluorescence Excitation Finite element analysis Electric fields Microscopes
Content Type	Text
Resource Type	Article

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