NDLI: Identification of pathogenic T cell epitopes near cathepsin cleavage sites in thyroglobulin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jiang, Hong Kolypetri, Panayota Carayanniotis, George
Description	Country affiliation: Canada Author Affiliation: Kolypetri P ( Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador A1B 3V6, Canada.)
Abstract	Experimental autoimmune thyroiditis, induced in mice after challenge with thyroglobulin (Tg), is known to be under the genetic control of the H2A(k) locus. Because cathepsins are known to influence proteolytic processing of Tg in vivo, we examined in this study whether putative H2A(k)-binding Tg epitopes, located near cathepsin cleavage sites within mouse Tg, have immunopathogenic properties. Cathepsin L, B, and D cleavage sites in mouse Tg were predicted based on homology with known cathepsin cleavage sites in rabbit Tg. We used an algorithm-based approach to identify H2A(k)-binding motifs within 20-aa residue segments adjacent to cathepsin cleavage sites, and five 12mer peptides encompassing these sequences were synthesized. Two of them, p2369 (aa 2369-2380) and p2439 (aa 2439-2450) were immunogenic, eliciting significant proliferative T cell responses using lymph node cells from peptide-primed mice and production of IL-2 and IFN-Î³ in recall assays in vitro. Both peptides induced experimental autoimmune thyroiditis upon direct challenge of CBA/J mice with peptide in CFA and by adoptive transfer of peptide-primed lymph node cells into naive recipient hosts, but neither peptide was characterized as dominant.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	4
Volume Number	190
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2013-02-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cathepsins Metabolism Epitopes, T-lymphocyte Toxicity Thyroglobulin Thyroiditis, Autoimmune Immunology Amino Acid Motifs Amino Acid Sequence Animals Cd4-positive T-lymphocytes Pathology Cathepsin B Cathepsin D Cathepsin L Cells, Cultured H-2 Antigens Genetics Mice Mice, Inbred Cba Mice, Inbred Icr Molecular Sequence Data Protein Binding Rabbits Structural Homology, Protein Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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