NDLI: Amphisomal route of MHC class I cross-presentation in bacteria-infected dendritic cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Weisser, Tanja Kägebein, Danny Sens, Mareen Knittler, Michael R. Liebler-Tenorio, Elisabeth M. Gutjahr, Melanie Fiegl, Dorothee
Description	Country affiliation: Germany Author Affiliation: Fiegl D ( Institute of Immunology, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institute, 17493 Greifswald-Isle of Riems, Germany.)
Abstract	Dendritic cells (DCs) are among the first professional APCs encountered by the obligate intracellular bacterium Chlamydia during infection. Using an established mouse bone marrow-derived DC line, we show that DCs control chlamydial infection in multiple small inclusions characterized by restricted bacterial growth, impaired cytosolic export of the virulence factor chlamydial protease-like activity factor, and interaction with guanylate-binding protein 1, a host cell factor involved in the initiation of autophagy. During maturation of infected DCs, chlamydial inclusions disintegrate, likely because they lack chlamydial protease-like activity factor-mediated protection. Released cytosolic Chlamydia are taken up by autophagosomes and colocalize with cathepsin-positive amphisomal vacuoles, to which peptide transporter TAP and upregulated MHC class I (MHC I) are recruited. Chlamydial Ags are subsequently generated through routes involving preprocessing in amphisomes via cathepsins and entry into the cytosol for further processing by the proteasome. Finally, bacterial peptides are reimported into the endosomal pathway for loading onto recycling MHC I. Thus, we unravel a novel pathway of MHC I-mediated cross-presentation that is initiated with a host cellular attack physically disrupting the parasitophorous vacuole, involves autophagy to collect cytosolic organisms into autophagosomes, and concludes with complex multistep antigenic processing in separate cellular compartments.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	6
Volume Number	190
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2013-03-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chlamydophila Psittaci Immunology Cross-priming Dendritic Cells Microbiology Histocompatibility Antigens Class I Animals Autophagy Bronchopneumonia Cd8-positive T-lymphocytes Metabolism Cell Line Cell Line, Transformed Cercopithecus Aethiops Pathology Mice Mice, Inbred C57bl Psittacosis Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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