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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gu, Tingting Bi, Shuyun Wang, Yu Wang, Tianjiao Pang, Bo Zhao, Tingting |
| Description | Country affiliation: China Author Affiliation: Zhao T ( College of Chemistry, Changchun Normal University, Changchun 130032, China.); Bi S ( College of Chemistry, Changchun Normal University, Changchun 130032, China. Electronic address: sy_bi@sina.com.); Wang Y ( College of Chemistry, Changchun Normal University, Changchun 130032, China.); Wang T ( College of Chemistry, Changchun Normal University, Changchun 130032, China.); Pang B ( TechnologyCenterofInspectionandQuarantine, Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China.); Gu T ( TechnologyCenterofInspectionandQuarantine, Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China.) |
| Abstract | The salmeterol xinafoate (SX) binding to calf thymus DNA in vitro was explored by fluorescence, resonance light scattering (RLS), UV-vis absorption, as well as viscometry, ionic strength effect and DNA melting techniques. It was found that SX could bind to DNA weakly, and the binding constants (Ka) were determined as 8.52×10(3), 8.31×10(3) and 6.14×10(3) L mol(-1) at 18, 28 and 38°C respectively. When bound to DNA, SX showed fluorescence quenching in the fluorescence spectra and hyperchromic effect in the absorption spectra. Stern-Volmer plots revealed that the quenching of fluorescence of SX by DNA was a static quenching. Furthermore, the relative viscosity and melting temperature of DNA solution were hardly influenced by SX, while the fluorescence intensity of SX-DNA was observed to decrease with the increasing ionic strength of system. Also, the binding constant between SX and double stranded DNA (dsDNA) was much weaker than that between SX and single stranded DNA (ssDNA). All these results suggested that the binding mode of SX to DNA should be groove binding. The obtained thermodynamic parameters indicated that electrostatic force might play a predominant role in SX binding to DNA. The quantum yield (φ) of SX was measured as 0.13 using comparative method. Based on the Förster resonance energy transfer theory (FRET), the binding distance (r0) between the acceptor and donor was calculated as 4.10 nm. |
| ISSN | 13861425 |
| Volume Number | 132 |
| e-ISSN | 18733557 |
| Journal | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-11-11 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Albuterol Analogs & Derivatives Dna Metabolism Chemistry Animals Binding Sites Cattle Hydrogen-ion Concentration Kinetics Light Nucleic Acid Denaturation Osmolar Concentration Salmeterol Xinafoate Scattering, Radiation Spectrometry, Fluorescence Temperature Thermodynamics Viscosity Journal Article Research Support, Non-u.s. Gov't Discipline Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Atomic and Molecular Physics, and Optics Analytical Chemistry Instrumentation |
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