| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Klibi, Naouel Ben Said, Leila Alonso, Carla Andrea Torres, Carmen Jouini, Ahlem Dziri, Raoudha Boudabous, Abdellatif Ben Slama, Karim |
| Spatial Coverage |
Tunisia |
| Description |
Country affiliation: Tunisia Author Affiliation: Ben Said L ( Laboratoire des Microorganismes et Biomolécules actives, Faculté de Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.); Jouini A ( Laboratory of Epidemiology and Veterinary Microbiology, Pasteur Institute of Tunis, Tunisia.); Alonso CA ( Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain.); Klibi N ( Laboratoire des Microorganismes et Biomolécules actives, Faculté de Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.); Dziri R ( Laboratoire des Microorganismes et Biomolécules actives, Faculté de Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.); Boudabous A ( Laboratoire des Microorganismes et Biomolécules actives, Faculté de Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.); Ben Slama K ( Laboratoire des Microorganismes et Biomolécules actives, Faculté de Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia); Torres C ( Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain. Electronic address: carmen.torres@unirioja.es.) |
| Abstract |
The presence of extended-spectrum beta-lactamase and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae (ESBL-Eb and pAmpC-Eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in Tunisia. Twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained ESBL-Eb or pAmpC-Eb; one ESBL/pAmpC-Eb per positive sample was further characterized. Beta-lactamase genes detected were as follows: blaCTX-M-1 (10 Escherichia coli),blaCTX-M-15 (eight E. coli, one Klebsiella pneumoniae, one Citrobacter freundii), blaCTX-M-14 (one E. coli) and blaCMY-2 (four E. coli). The blaTEM-1, blaOXA-1 or blaSHV-1 genes were also found in 72% of these isolates. The ISEcp1, orf477 or IS903 sequences were found upstream or downstream of blaCTX-M genes. Class 1 integrons were present in 16 of the 25 ESBL-Eb/pAmpC-Eb strains (64%), and contained five different gene-cassette arrays. Most of the strains (76%) showed a multiresistant phenotype and qnr genes were identified in four strains. Molecular typing of ESBL/CMY-2-producing E. coli isolates showed 23 different PFGE-patterns and 15 different sequence-types (ST10, ST46, ST48, ST58, ST69, ST101, ST117, ST131, ST141, ST288, ST359, ST399, ST405, ST617, and the new ST4530); these strains were ascribed to phylogroups A (11 isolates), B1 (3 isolates), D (6 isolates) and B2 (3 isolates). From one to five plasmids were detected in each strain (size from 30kb to >240kb) and ESBL or pAmpC genes were transferred by conjugation in 69.5% of the E. coli strains. In conclusion, ESBL-Eb and pAmpC-Eb strains are frequently detected in wastewater samples and they might be a source for dissemination in other environments with repercussion in public health. |
| ISSN |
00489697 |
| Volume Number |
550 |
| e-ISSN |
18791026 |
| Journal |
Science of The Total Environment |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-04-15 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Bacterial Proteins Metabolism Enterobacteriaceae Growth & Development Environmental Monitoring Water Microbiology Water Pollutants, Chemical Analysis Beta-lactamases Drug Resistance, Bacterial Genetics Genes, Bacterial Tunisia Journal Article Research Support, Non-u.s. Gov't Discipline Environmental Science |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Environmental Chemistry Waste Management and Disposal Pollution Environmental Engineering |
