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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sinz, Andrea Arlt, Christian Chorev, Dror Sharon, Michal |
| Description | Country affiliation: Germany Author Affiliation: Sinz A ( Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, D-06120, Halle, Germany.); Arlt C ( Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, D-06120, Halle, Germany.); Chorev D ( Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel.); Sharon M ( Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel.) |
| Abstract | Mass spectrometry (MS) is becoming increasingly popular in the field of structural biology for analyzing protein three-dimensional-structures and for mapping protein-protein interactions. In this review, the specific contributions of chemical crosslinking and native MS are outlined to reveal the structural features of proteins and protein assemblies. Both strategies are illustrated based on the examples of the tetrameric tumor suppressor protein p53 and multisubunit vinculin-Arp2/3 hybrid complexes. We describe the distinct advantages and limitations of each technique and highlight synergistic effects when both techniques are combined. Integrating both methods is especially useful for characterizing large protein assemblies and for capturing transient interactions. We also point out the future directions we foresee for a combination of in vivo crosslinking and native MS for structural investigation of intact protein assemblies. |
| ISSN | 09618368 |
| e-ISSN | 1469896X |
| DOI | 10.1002/pro.2696 |
| Journal | Protein Science |
| Issue Number | 8 |
| Volume Number | 24 |
| Language | English |
| Publisher | Wiley-Blackwell (on behalf of The Protein Society) |
| Publisher Date | 2015-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cross-linking Reagents Chemistry Mass Spectrometry Protein Interaction Mapping Proteins Metabolism Animals Models, Molecular Protein Conformation Proteomics Tumor Suppressor Protein P53 Discipline Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Biochemistry |
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