| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Wang, Xu He, Songbing Yu, Jing Wang, Lei Zhu, Xinguo Han, Ye Hu, You Liu, Pengfei |
| Description |
Author Affiliation: Yu J ( Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.); He S ( Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.); Liu P ( Department of Gastrointestinal Surgery, The First People's Hospital of Wujiang City, Wujiang Hospital Afï¬ liated to Nantong University, Wujiang, Jiangsu 215200, P.R. China.); Hu Y ( Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.); Wang L ( Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.); Wang X ( Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.); Han Y ( Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.); Zhu X ( Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.) |
| Abstract |
Patients with ulcerative colitis (UC) are at increased risk of developing colitisassociated colon cancer. Previous studies have indicated that interleukin (IL)21, which is predominantly secreted by follicular T helper (Tfh) cells, is overproduced in inflammatory bowel diseases. In order to investigate the role of IL21 in UC and the association between IL21 and Tfh cells, the number of Tfh cells and the level of IL21 were investigated in colonic tissues from UC patients and wildtype (WT) mice, which were induced by dextran sulphate sodium (DSS). High Tfh cell counts and levels of IL21 were observed in UC patients and WT mice with DSSinduced colitis. Subsequent comparison of the mucosal damage and expression of Tfhassociated cytokines in the WT mice and IL21 knockout (IL21KO) mice following DSS administration, revealed that IL21KO mice were largely protected against colitis and exhibited reduced infiltration of Tfh cells, as well as decreased production of Tfhassociated cytokines. The present study also found that IL21 was necessary for the proliferation and secretion of Tfh cells in vitro. In addition, neutralization of IL21 in DSSadministered WT mice using antiIL21 reduced the number of Tfh cells and the level of mucosal damage. Administration of a neutralizing IL21 antibody decreased the colonic infiltration of Tfh cells and reduced damage to the mucosa. These results indicated that Tfh cells are important in UC and that its effector molecule, IL21, is not only a critical regulator of inflammation, but also regulates the proliferation and response of Tfh cells in the colitis microenvironment. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
2 |
| Volume Number |
11 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-02-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Interleukins Metabolism T-lymphocytes, Helper-inducer Animals Antibodies, Neutralizing Pharmacology Cd40 Ligand Genetics Cell Proliferation Drug Effects Cells, Cultured Cellular Microenvironment Colitis, Ulcerative Chemically Induced Pathology Colon Dextran Sulfate Toxicity Disease Models, Animal Inducible T-cell Co-stimulator Protein Mice Mice, Inbred C57bl Mice, Knockout Programmed Cell Death 1 Receptor Proto-oncogene Proteins C-bcl-6 Receptors, Cxcr5 Cytology Secretion Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
