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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xu, Hai Shan, Honghong Lu, Jian R. Wang, Xiaoqiang Wang, Yuming |
| Description | Country affiliation: China Author Affiliation: Wang X ( Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao Economic Development Zone, Qingdao 266555, China.) |
| Abstract | Spectroscopic ellipsometry (SE) and atomic force microscopy (AFM) have been used to investigate the adsorption of a mouse monoclonal antibody (type IgG1, anti-beta-hCG) on hydrophilic silica (bearing weak negative charges above pH 3), followed by the assessment of binding of human chorionic gonadotrophin (hCG). The antibody is a relatively large molecule with a molecular weight of 150 kDa and the isoelectric point (IP) around pH 6. The antibody adsorption was conducted at pH 4.0, 6.0 and 8.0 to examine the role of charge interaction. Ellipsometric results show that away from the IP, both initial adsorption rate and surface excess decreased, with the reduction at pH 8.0 being more pronounced than that at pH 4.0 due to the electrostatic repulsion not only between the charged antibody molecules within the adsorbed layer but also between antibody and the silica surface. Whilst parallel AFM measurements confirmed the main trend of pH dependent antibody adsorption, they also revealed the tendency of surface aggregation with increasing surface coverage. AFM height profiling at low surface coverage confirmed the 'flat-on' orientation of adsorbed antibody molecules, consistent with the previous study by neutron reflection. Interestingly, the antibody height at pH 4.0 was found to be lower than that at pH 8.0, showing the influence from different electrostatic interactions under the two pH conditions. Subsequent hCG binding to the adsorbed antibodies was found to decrease with increasing surface coverage due to the steric hindrance. Under similar antibody surface coverage, the hCG binding ratio at pH 8 was higher than that at pH 4.0, a difference that could only be accounted for by the tighter surface confinement at pH 4.0. |
| ISSN | 00219797 |
| Issue Number | 1 |
| Volume Number | 323 |
| e-ISSN | 10957103 |
| Journal | Journal of Colloid and Interface Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-07-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Adsorption Antibodies, Monoclonal Chemistry Microscopy, Atomic Force Methods Silicon Dioxide Spectrophotometry Animals Biocompatible Materials Chorionic Gonadotropin Metabolism Humans Hydrogen-ion Concentration Materials Testing Mice Models, Biological Surface Properties Time Factors Journal Article Discipline Colloid & Interface Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surfaces, Coatings and Films Colloid and Surface Chemistry Biomaterials Electronic, Optical and Magnetic Materials |
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