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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Liu, Chuen-Ying Misra, Tarun Kumar Huang, Kuan-Pin |
| Description | Country affiliation: Taiwan Author Affiliation: Misra TK ( Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.) |
| Abstract | Water-dispersed, spherical, underivatized Au-NPs with particle size less than 5 nm were synthesized from an aqueous solution of 32-macrocyclic polyammonium chloride, [32]ane-(NH(2)(+))(8).8Cl(-) (32-MCPAC) using sodium borohydride (NaBH(4)) as the reducing agent. The bioconjugation of the synthesized Au-NPs at different pHs (3.6-5.6) with bovine serum albumin (BSA) protein was studied using UV-Vis, fluorescence, and Raman spectroscopy. These studies support that the Au-NPs were incorporated into the protein moiety and bound to it chemically. The binding constants (K(b)) and stoichiometries (n) (i.e., the number of Au-NPs bound by the proteins) of BSA protein to the Au-NPs at different pHs were determined by measuring the quenching of the fluorescence intensity of the tryptophan residues of the protein molecules after conjugation. The values for K(b) (n) were found to be 1.05 x 10(10) M(-1) (1.66), 2.09 x 10(10) M(-1) (2.30), and 1.86 x 10(10) M(-1) (1.75) at pH 3.60, 4.60, and 5.60 for BSA-Au-NPs conjugations, respectively. The results show that BSA binds to the Au-NPs strongly at pH 4.60, which is equivalent to its isoelectric point (pI 4.6). |
| ISSN | 00219797 |
| Issue Number | 1 |
| Volume Number | 344 |
| e-ISSN | 10957103 |
| Journal | Journal of Colloid and Interface Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-04-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Gold Chemistry Macrocyclic Compounds Nanoparticles Quaternary Ammonium Compounds Serum Albumin, Bovine Animals Cations Cattle Ultrastructure Spectrometry, Fluorescence Spectrophotometry Spectrum Analysis, Raman Journal Article Research Support, Non-u.s. Gov't Discipline Colloid & Interface Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surfaces, Coatings and Films Colloid and Surface Chemistry Biomaterials Electronic, Optical and Magnetic Materials |
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