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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Deep, Shashank Sharma, Anurag Agarwal, Pratibha K. |
| Description | Country affiliation: India Author Affiliation: Sharma A ( Department of Chemistry, Indian Institute of Technology, Delhi 110016, India.) |
| Abstract | To characterize the structural changes in bovine serum albumin (BSA) on the addition of N-cetyl-N,N,N-trimethyl ammonium bromide (CTAB) and to understand the mechanism underlying aggregation of resulting protein-surfactant complex, UV-visible absorbance, steady-state fluorescence, SDS-PAGE gel electrophoresis, dynamic light scattering (DLS), and circular dichroism measurements of BSA-CTAB solutions were carried out. Changes in UV-visible absorbance and the fluorescence intensity on the addition of CTAB indicate the binding of CTAB to BSA. Changes in SDS-gel pattern of pepsin digested BSA sample, circular dichroism band at 222 nm, ANS intensity of BSA-ANS solution and hydrodynamic diameter of native state monomer on the addition of CTAB indicate that BSA unfolds in the presence of CTAB. Changes in the heme binding profile, temperature dependence of the heme binding profile and change from mixed to dynamic quenching in acrylamide quenching experiments of BSA solution on the addition of CTAB indicate that BSA unfolds to an intermediate. Turbidity and DLS measurements at 298 K, 323 K and 358 K indicate that the order of propensity to aggregate for different conformations of protein is: denatured > intermediate > native. The formation of aggregates is influenced by the nature of head and tail of the surfactant. |
| ISSN | 00219797 |
| Issue Number | 2 |
| Volume Number | 343 |
| e-ISSN | 10957103 |
| Journal | Journal of Colloid and Interface Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-03-15 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Monosaccharides Chemistry Quaternary Ammonium Compounds Serum Albumin, Bovine Animals Cattle Circular Dichroism Electrophoresis, Polyacrylamide Gel Particle Size Protein Folding Journal Article Research Support, Non-u.s. Gov't Discipline Colloid & Interface Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surfaces, Coatings and Films Colloid and Surface Chemistry Biomaterials Electronic, Optical and Magnetic Materials |
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