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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Durand, Dominique Balakrishnan, Gireeshkumar Nicolai, Taco |
| Description | Country affiliation: France Author Affiliation: Balakrishnan G ( LUNAM Université du Maine, IMMM, PCI, UMR-CNRS, 72085 le Mans Cedex 9, France.) |
| Abstract | Diffusion of fluorescent-labeled dextran with different molecular weights was investigated in ß-lactoglobulin (ß-lg) solutions and gels over a wide range of salt and protein concentrations at pH 7 by combining confocal laser scanning microscope (CLSM) with fluorescence recovery after photobleaching (FRAP). Effects of the protein concentration, the salt concentration and the tracer size were investigated in detail. Diffusion in turbid heterogeneous gels formed at 0.2M NaCl depended weakly on the probe size and the protein concentration and remained close to that in unheated solutions. A strong decrease of the diffusion coefficient with increasing tracer size and protein concentration was observed in more homogeneous gels formed at lower salt concentrations. Larger dextran chains were trapped in transparent gels formed at NaCl concentration below 0.1M. The present investigation complements an earlier study of tracer diffusion of larger spherical probes in ß-lg gels using multi-particle tracking. |
| ISSN | 00219797 |
| Issue Number | 1 |
| Volume Number | 388 |
| e-ISSN | 10957103 |
| Journal | Journal of Colloid and Interface Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-12-15 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Anticoagulants Metabolism Dextrans Gels Chemistry Lactoglobulins Fluorescence Recovery After Photobleaching Hydrogen-ion Concentration Ultrastructure Microscopy, Confocal Sodium Chloride Pharmacology Journal Article Discipline Colloid & Interface Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surfaces, Coatings and Films Colloid and Surface Chemistry Biomaterials Electronic, Optical and Magnetic Materials |
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