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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Lifeng Yue, Junqi Wu, Jingming Yang, Zhaoguang Hu, Ruikang |
| Description | Country affiliation: Singapore Author Affiliation: Wu J ( Center for Advanced Water Technology, The Toh Tuck Complex, 80/82, Toh Guan Road East, Singapore 608575, Singapore.) |
| Abstract | Injection-port derivatization combined with solid-phase extraction (SPE) was developed and applied for the first time to determine five types of fecal sterols (coprostanol, cholestanol, epicholestanol, epicoprostanol and cholesterol) with gas chromatography-mass spectrometry (GC-MS). In this method, silylation of fecal sterols was performed with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) at GC injection-port. The factors influential to this technique such as injection-port temperature, purge-off time, derivatization reagent (BSTFA) volume, and the type of organic solvent were investigated. In addition, the conditions of SPE (including the type of SPE cartridge, the type of elution organic solvent) were also studied. After SPE followed by injection-port silylation by GC-MS, good linearity of analytes was achieved in the range of 0.02-10ng/mL with coefficients of determination, R(2)>0.995. Good reproducibility was obtained with relative standard deviation less than 19.6%. The limits of detection ranged from 1.3ng/mL to 15ng/mL (S/N=3) in environmental water samples. Compared with traditional off-line silylation of fecal sterols performed with water bath (60 degrees C, 30min), this injection-port silylation method is much simpler and convenient. The developed method has been successfully applied for the analysis of fecal sterols from real environmental water samples. |
| ISSN | 00219673 |
| Issue Number | 7 |
| Volume Number | 1216 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-02-13 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cholestanols Analysis Cholesterol Feces Chemistry Gas Chromatography-mass Spectrometry Methods Solid Phase Extraction Animals Dogs Humans Mice Reproducibility Of Results Solvents Water Supply Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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