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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Atsuta, Yoichi Daimon, Hiroyuki Hanif, Muhammad Fujie, Koichi |
| Description | Country affiliation: Japan Author Affiliation: Hanif M ( Department of Ecological Engineering, Toyohashi University of Technology, Aichi 441-8580, Japan.) |
| Abstract | Supercritical carbon dioxide (scCO2) extraction was applied for the determination of microbial phospholipid fatty acids (PLFA) in activated sludge. Quantification was performed by using gas chromatography-mass spectrometry (GC-MS). The highest extraction yields of PLFA, at a concentration of 7.28 nmol/mg-dry activated sludge, was obtained at a temperature of 80°C, pressure of 25 MPa and 10% (v/v) methanol for a 15-min extraction time. ScCO2 extraction results obtained in these conditions were comparable with those obtained by liquid organic solvent extraction (LSE) based on diversity and equalibility indices. The repeatability test showed that the relative standard deviation values were less than 13%. The experimental results show that the scCO2 extraction saves time and uses much less organic solvent. In addition, scCO2 extraction is a promising and alternative method for the analysis of microbial community structure in environmental assessment using the PLFA profile. |
| ISSN | 00219673 |
| Issue Number | 43 |
| Volume Number | 1217 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-10-22 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, Supercritical Fluid Methods Fatty Acids Isolation & Purification Gas Chromatography-mass Spectrometry Phospholipids Sewage Chemistry Microbiology Carbon Dioxide Methanol Pressure Reproducibility Of Results Solvents Temperature Comparative Study Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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