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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Eeltink, Sebastiaan Ursem, Mario Schoenmakers, Peter J. Dolman, Sebastiaan Swart, Remco Detobel, Frederik |
| Description | Country affiliation: Belgium Author Affiliation: Eeltink S ( Vrije Universiteit Brussel, Department of Chemical Engineering, Brussels, Belgium. seeltink@vub.ac.be) |
| Abstract | In this study, high-efficiency LC-MS/MS separations of complex proteolytic digests are demonstrated using 50 mm, 250 mm, and 1m long poly(styrene-co-divinylbenzene) monolithic capillary columns. The chromatographic performance of the 50 and 250 mm monoliths was compared at the same gradient steepness for gradient durations between 5 and 150 min. The maximum peak capacity of 400 obtained with a 50mm column, increased to 485 when using the 250 mm long column and scaling the gradient duration according column length. With a 5-fold increase in column length only a 20% increase in peak capacity was observed, which could be explained by the larger macropore size of the 250 mm long monolith. When taking into account the total analysis time, including the dwell time, gradient time and column equilibration time, the 50mm long monolith yielded better peptide separations than the 250 mm long monolithic column for gradient times below 80 min (n(c)=370). For more demanding separation the 250 mm long monolith provided the highest peak production rate and consequently higher sequence coverage. For the analysis of a proteolytic digest of Escherichia coli proteins a monolithic capillary column of 1m in length was used, yielding a peak capacity of 1038 when applying a 600 min gradient. |
| ISSN | 00219673 |
| Issue Number | 43 |
| Volume Number | 1217 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-10-22 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, Liquid Instrumentation Methods Peptide Fragments Isolation & Purification Proteomics Tandem Mass Spectrometry Escherichia Coli Proteins Microscopy, Electron, Scanning Polystyrenes Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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