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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Venäläinen, Tuula Tammi, Markku Oikari, Sanna |
| Description | Author Affiliation: Oikari S ( Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland); Venäläinen T ( Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.); Tammi M ( Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.) |
| Abstract | In this paper we describe a method optimized for the purification of uridine diphosphate (UDP)-sugars from liver, adipose tissue, brain, and heart, with highly reproducible up to 85% recoveries. Rapid tissue homogenization in cold ethanol, lipid removal by butanol extraction, and purification with a graphitized carbon column resulted in isolation of picomolar quantities of the UDP-sugars from 10 to 30mg of tissue. The UDP-sugars were baseline separated from each other, and from all major nucleotides using a CarboPac PA1 anion exchange column eluted with a gradient of acetate and borate buffers. The extraction and purification protocol produced samples with few unidentified peaks. UDP-N-acetylglucosamine was a dominant UDP-sugar in all the rat tissues studied. However, brain and adipose tissue showed high UDP-glucose levels, equal to that of UDP-N-acetylglucosamine. The UDP-N-acetylglucosamine showed 2.3-2.7 times higher levels than UDP-N-acetylgalactosamine in all tissues, and about the same ratio was found between UDP-glucose and UDP-galactose in adipose tissue and brain (2.6 and 2.8, respectively). Interestingly, the UDP-glucose/UDP-galactose ratio was markedly lower in liver (1.1) and heart (1.7). The UDP-N-acetylglucosamine/UDP-glucuronic acid ratio was also constant, between 9.7 and 7.7, except in liver with the ratio as low as 1.8. The distinct UDP-glucose/galactose ratio, and the abundance of UDP-glucuronic acid may reflect the specific role of liver in glycogen synthesis, and metabolism of hormones and xenobiotics, respectively, using these UDP-sugars as substrates. |
| ISSN | 00219673 |
| Volume Number | 1323 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-01-03 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Adipose Tissue Chemistry Borates Brain Chemistry Liver Myocardium Uridine Diphosphate Sugars Isolation & Purification Animals Anions Chromatography, High Pressure Liquid Chromatography, Ion Exchange Male Rats Uridine Diphosphate Galactose Analysis Uridine Diphosphate N-acetylglucosamine Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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