| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Liu, Yilan Zhou, Jiemin Huo, Weiyan Yang, Maohua Chen, Jinjin Chen, Ting Wang, Caixia Xing, Jianmin Yan, Daojiang |
| Description |
Country affiliation: China Author Affiliation: Liu Y ( National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China); Chen T ( Department of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.); Yang M ( National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.); Wang C ( National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China); Huo W ( Huabei oilfield No. 1 middle school, Renqiu 062550, Cangzhou City, Hebei Province, China.); Yan D ( National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China); Chen J ( National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China); Zhou J ( National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China); Xing J ( National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: jmxing@home.ipe.ac.cn.) |
| Abstract |
Microbial production of fatty acids and fatty alcohols has attracted increasing concerns because of energy crisis and environmental impact of fossil fuels. Therefore, simple and efficient methods for the extraction and quantification of these compounds become necessary. In this study, a high-performance liquid chromatography-refractive index detection (HPLC-RID) method was developed for the simultaneous quantification of fatty acids and fatty alcohols in these samples. The optimum chromatographic conditions are C18 column eluted with methanol:water:acetic acid (90:9.9:0.1, v/v/v); column temperature, 26°C; flow rate, 1.0mL/min. Calibration curves of all selected analytes showed good linearity (r(2)≥0.9989). The intra-day and inter-day relative standard deviations (RSDs) of the 10 compounds were less than 4.46% and 5.38%, respectively, which indicated that the method had good repeatability and precision. Besides, a method for simultaneous extraction of fatty acids and fatty alcohols from fermentation broth was optimized by orthogonal design. The optimal extraction conditions were as follows: solvent, ethyl acetate; solvent to sample ratio, 0.5:1; rotation speed, 2min at 260rpm; extraction temperature, 10°C. This study provides simple and fast methods to simultaneously extract and quantify fatty acids and fatty alcohols for the first time. It will be useful for the study of microbial production of these products. |
| ISSN |
00219673 |
| Volume Number |
1323 |
| e-ISSN |
18733778 |
| Journal |
Journal of Chromatography A |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-01-03 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Fatty Acids Analysis Fatty Alcohols Fermentation Calibration Chromatography, High Pressure Liquid Economics Escherichia Coli Metabolism Limit Of Detection Pseudomonas Aeruginosa Time Factors Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Organic Chemistry Medicine Analytical Chemistry Biochemistry |
