| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Lendoiro, Elena López-Rivadulla, Manuel de Castro, Ana Cruz, Angelines Fernández-Vega, Hadriana Steinmeyer, Stefan |
| Description |
Author Affiliation: de Castro A ( Servicio de Toxicología Forense, Instituto de Ciencias Forenses, Universidad de Santiago de Compostela, San Francisco s/n, 15782 Santiago de Compostela, Spain. Electronic address: ana.decastro@usc.es.); Lendoiro E ( Servicio de Toxicología Forense, Instituto de Ciencias Forenses, Universidad de Santiago de Compostela, San Francisco s/n, 15782 Santiago de Compostela, Spain. Electronic address: elena.lendoiro@usc.es.); Fernández-Vega H ( Servicio de Toxicología Forense, Instituto de Ciencias Forenses, Universidad de Santiago de Compostela, San Francisco s/n, 15782 Santiago de Compostela, Spain. Electronic address: hadrianoia@hotmail.com.); Steinmeyer S ( Drug Testing Solutions & Forensic Applications, Dräger Safety AG & Co. KGaA, Lübeck, Germany. Electronic address: stefan.Steinmeyer@draeger.com.); López-Rivadulla M ( Servicio de Toxicología Forense, Instituto de Ciencias Forenses, Universidad de Santiago de Compostela, San Francisco s/n, 15782 Santiago de Compostela, Spain. Electronic address: manuel.lopez-rivadulla@usc.es.); Cruz A ( Servicio de Toxicología Forense, Instituto de Ciencias Forenses, Universidad de Santiago de Compostela, San Francisco s/n, 15782 Santiago de Compostela, Spain. Electronic address: angelines.cruz@usc.es.) |
| Abstract |
Since the past few years, several synthetic cathinones and piperazines have been introduced into the drug market to substitute illegal stimulant drugs such as amphetamine and derivatives or cocaine due to their unregulated situation. These emerging drugs are not usually included in routine toxicological analysis. We developed and validated a LC-MS/MS method for the determination of methedrone, methylone, mephedrone, 3,4-methylenedioxypyrovalerone (MDPV), fluoromethcathinone, fluoromethamphetamine, 1-(3-chlorophenyl)piperazine (mCPP) and 3-trifluoromethylphenylpiperazine (TFMPP) in oral fluid. Sample extraction was performed using Strata X cartridges. Chromatographic separation was achieved in 10min using an Atlantis(®) T3 column (100mm×2.1mm, 3µm), and formic acid 0.1% and acetonitrile as mobile phase. The method was satisfactorily validated, including selectivity, linearity (0.2-0.5 to 200ng/mL), limits of detection (0.025-0.1ng/mL) and quantification (0.2-0.5ng/mL), imprecision and accuracy in neat oral fluid (%CV=0.0-12.7% and 84.8-103.6% of target concentration, respectively) and in oral fluid mixed with Quantisal™ buffer (%CV=7.2-10.3% and 80.2-106.5% of target concentration, respectively), matrix effect in neat oral fluid (-11.6 to 399.7%) and in oral fluid with Quantisal™ buffer (-69.9 to 131.2%), extraction recovery (87.9-134.3%) and recovery from the Quantisal™ (79.6-107.7%), dilution integrity (75-99% of target concentration) and stability at different conditions (-14.8 to 30.8% loss). In addition, cross reactivity produced by the studied synthetic cathinones in oral fluid using the Dräger DrugTest 5000 was assessed. All the analytes produced a methamphetamine positive result at high concentrations (100 or 10µg/mL), and fluoromethamphetamine also at low concentration (0.075µg/mL). |
| ISSN |
00219673 |
| Volume Number |
1374 |
| e-ISSN |
18733778 |
| Journal |
Journal of Chromatography A |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-12-29 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Alkaloids Analysis Chromatography, High Pressure Liquid Methods Piperazines Tandem Mass Spectrometry Chemical Synthesis Calibration Cross Reactions Humans Immunoassay Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Organic Chemistry Medicine Analytical Chemistry Biochemistry |
