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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ma, Ying Tong, Shengqiang Ito, Yoichiro |
| Description | Country affiliation: United States Author Affiliation: Tong S ( College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, China); Ito Y ( Laboratory of Bioseparation Technologies, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: itoy@nhlbi.nih.gov.); Ma Y ( Laboratory of Molecular Imaging and Nanomedicine, National Institutes of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.) |
| Abstract | Spiral tube assembly counter-current chromatography was successfully applied in enantioseparation of dl-tryptophan using bovine serum albumin as chiral selector. An improved biphasic aqueous-aqueous solvent system 12.0% (w/w) polyethyleneglycol 8000-9.0% (w/w) dibasic potassium phosphate-0.1% ammonia-78.9% water was used as the solvent system for counter-current chromatography, in which bovine serum albumin was predominantly distributed in the lower phase of the two-phase aqueous system. The aqueous-aqueous solvent system gave a very high enantioselectivity for d- and l-tryptophan at =2.605 along with distribution ratio DD=1.200 and DL=0.461. High peak resolution was obtained for enantioseparation of 2.0mg of dl-tryptophan by spiral tube assembly counter-current chromatography under room temperature. It was found that 0.1% ammonia added in the aqueous-aqueous solvent system greatly improved the enantioseparations. An unusual extremely broad peak for l-tryptophan was observed during enantioseparations. In order to give an explanation, mass transfer rates of d- and l-enantiomers through the interface between the two phases were measured. It was found that l-tryptophan showed lower mass transfer rate than d-tryptophan. Further discussions were proposed for possible reasons for mass transfer rate difference between the enantiomers. |
| ISSN | 00219673 |
| e-ISSN | 18733778 |
| DOI | 10.1016/j.chroma.2014.11.013 |
| Journal | Journal of Chromatography A |
| Volume Number | 1374 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-12-29 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Chromatography, High Pressure Liquid Countercurrent Distribution Tryptophan Isolation & Purification Animals Liquid-liquid Extraction Serum Albumin, Bovine Solvents Chemistry Stereoisomerism Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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