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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Stanová, Andrea Vojs Marák, Jozef Hrenáková, Martina |
| Description | Author Affiliation: Stanová AV ( Department of Analytical Chemistry, Faculty of Natural Science, Comenius University in Bratislava, Mlynská Dolina CH-2, 84215 Bratislava, Slovak Republic. Electronic address: stanova@fns.uniba.sk.); Hrenáková M ( Department of Analytical Chemistry, Faculty of Natural Science, Comenius University in Bratislava, Mlynská Dolina CH-2, 84215 Bratislava, Slovak Republic.); Marák J ( Department of Analytical Chemistry, Faculty of Natural Science, Comenius University in Bratislava, Mlynská Dolina CH-2, 84215 Bratislava, Slovak Republic.) |
| Abstract | Some potential problems that can occur during the analyses of complex samples by on-line combination of capillary isotachophoresis-capillary zone electrophoresis (cITP-CZE) in automated electrophoretic analyzer with the column-coupling configuration of the separation unit were studied in this work. The main focus was devoted on the reproducibility of important analytes' parameters (migration time, peak height and peak area) and also on the stability studies of selected low and high molecular mass analytes of inorganic/organic origins (bromate, vitamins, proteins) present at low concentration levels in different kinds of matrices (mineral water, human urine). Such study was carried out for the first time for the electrophoretic analyzer operating in the hydrodynamically closed separation system provided with contact-less conductivity detectors and UV detector in CZE step. Hydrodynamic and electroosmotic flows of the buffer solutions were suppressed and therefore, only the electrophoretic transport of ions was significant. Obtained results showed the different stabilities of the analytes and samples depending on their origin. The focus in the long-term analyses should be paid on the storage of the samples and on the regular changing the contents of electrolyte vessels to keep the electrolyte composition and separation conditions as constant as possible. |
| ISSN | 00219673 |
| Volume Number | 1392 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Electrophoresis, Capillary Methods Bromates Analysis Electroosmosis Humans Hydrodynamics Isotachophoresis Mineral Waters Muramidase Proteins Reproducibility Of Results Serum Albumin Urinalysis Vitamin B 6 Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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