| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
An, Mingrui Chang, Yan-Xu Tan, Zhijing Xu, Jing-Jing Peng, Li-Qing Li, Qin Yang, Rui Hao, Jie Cao, Jun Hu, Shuai-Shuai |
| Description |
Country affiliation: China Author Affiliation: Peng LQ ( College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.); Li Q ( Institute of Matetia Medica, Zhejiang Acdemy of Medical Sciences, Hangzhou 310013, China.); Chang YX ( Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. Electronic address: tcmcyx@126.com.); An M ( Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.); Yang R ( Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.); Tan Z ( Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.); Hao J ( Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, United States.); Cao J ( College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.); Xu JJ ( College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.); Hu SS ( College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.) |
| Abstract |
A simple, efficient and low-cost method based on matrix solid-phase dispersion (MSPD) microextraction and ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS) was developed for the determination of seven main natural phenols (gallic acid, hydroxytyrosol, methyl gallate, luteolin 7-O-ß-d-glucoside, rutin, ellagic acid and oleuropein) and other eleven compounds in olive fruits. The experimental conditions for the MSPD extraction, including the type of adsorbent, the amount of dispersing sorbent, the grinding time, and the type of elution solvent were investigated and optimized. The optimized parameters were determined to be that middle-molecular-weight chitosan was used as adsorbent, the amount of middle-molecular-weight chitosan was selected to be 25mg, the grinding time was chosen to be 60s, and methanol: water (6:4, v:v) was used as elution solvent. Compared with reported methods, the proposed method was more simple, rapid, and efficient. Moreover, this method required less extraction time and less amount of sample and solvent. The method showed good linearity (r(2)≥0.9909) for the seven analytes, with the limits of detection in the range of 69.6-358.4ng/g. And recoveries were above 80.06%. The methodology was successfully applied to the extraction and determination of seven phenolic compounds in olive fruitsï¼ Canarii fructusï¼ . |
| ISSN |
00219673 |
| Journal |
Journal of Chromatography A |
| Volume Number |
1456 |
| e-ISSN |
18733778 |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-07-22 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Analytical Chemistry |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Organic Chemistry Medicine Analytical Chemistry Biochemistry |
