NDLI: Nanoscale topography-induced modulation of fundamental cell behaviors of rabbit corneal keratocytes, fibroblasts, and myofibroblasts.

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Nanoscale topography-induced modulation of fundamental cell behaviors of rabbit corneal keratocytes, fibroblasts, and myofibroblasts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nealey, Paul F. Pot, Simon A. Liliensiek, Sara J. Murphy, Christopher J. Myrna, Kathern E. Bentley, Ellison Jester, James V.
Description	Author Affiliation: Pot SA ( Department of Surgical Sciences, School of Veterinary Medicine, and.)
Abstract	PURPOSE: Keratocyte-to-myofibroblast differentiation is a key factor in corneal wound healing. The purpose of this study was to determine the influence of environmental nanoscale topography on keratocyte, fibroblast, and myofibroblast cell behavior. METHODS: Primary rabbit corneal keratocytes, fibroblasts, and myofibroblasts were seeded onto planar polyurethane surfaces with six patterned areas, composed of anisotropically ordered grooves and ridges with a 400-, 800-, 1200-, 1600-, 2000-, and 4000-nm pitch (pitch = groove + ridge width). After 24 hours cells were fixed, stained, imaged, and analyzed for cell shape and orientation. For migration studies, cells on each patterned surface were imaged every 10 minutes for 12 hours, and individual cell trajectories and migration rates were calculated. RESULTS: Keratocytes, fibroblasts, and myofibroblasts aligned and elongated to pitch sizes larger than 1000 nm. A lower limit to the topographic feature sizes that the cells responded to was identified for all three phenotypes, with a transition zone around the 800- to 1200-nm pitch size. Fibroblasts and myofibroblasts migrated parallel to surface ridges larger than 1000 nm but lacked directional guidance on submicron and nanoscale topographic features and on planar surfaces. Keratocytes remained essentially immobile. CONCLUSIONS: Corneal stromal cells elongated, aligned, and migrated, differentially guided by substratum topographic features. All cell types failed to respond to topographic features approximating the dimensions of individual stromal fibers. These findings contribute to our understanding of corneal stromal cell biology in health and disease and their interaction with biomaterials and their native extracellular matrix.
ISSN	01460404
e-ISSN	15525783
DOI	10.1167/iovs.09-4074
Journal	Investigative Opthalmology & Visual Science
Issue Number	3
Volume Number	51
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2010-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Polarity Physiology Cell Shape Corneal Stroma Cytology Fibroblasts Actins Metabolism Animals Cell Culture Techniques Cell Movement Fluorescent Antibody Technique, Indirect Rabbits Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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