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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kamiya, Akira Ando, Joji Ishibazawa, Akihiro Yoshida, Akitoshi Yamamoto, Kimiko Nagaoka, Taiji Takahashi, Tatsuhisa |
| Description | Country affiliation: Japan Author Affiliation: Ishibazawa A ( Department of Ophthalmology, Asahikawa Medical University, Asahikawa, Japan.) |
| Abstract | PURPOSE: Physiological shear stress is higher in the retinal microcirculatory network than in other organs. The effects of laminar shear stress on gene expression in human retinal microvascular endothelial cells (HRMECs) was investigated. METHODS: Cultured HRMECs on glass plates were exposed to a laminar shear stress of 0, 1.5, 6, 15, 30, 60, or 100 dyne/cm(2) for 24 hours and to 60 dyne/cm(2) for 0, 1, 3, 6, 12, 24, or 48 hours. The mRNA expressions of endothelial nitric oxide synthase (eNOS), endothelin-1 (ET-1), and thrombomodulin (TM) in the HRMECs were evaluated using real-time reverse transcription polymerase chain reaction. RESULTS: The HRMECs elongated and aligned parallel with the flow direction based on the shear stress and exposure times. The expression of eNOS mRNA gradually increased and became saturated at 60 dyne/cm(2); ET-1 mRNA expression increased at 1.5 dyne/cm(2) but decreased below that of the static control at shear stresses of 30 dyne/cm(2) or more. TM mRNA expression in response to shear stress increased linearly from 0 to 100 dyne/cm(2). A shear stress of 60 dyne/cm(2) for 6 hours or more promoted eNOS and TM mRNA expression but suppressed ET-1 mRNA expression in HRMECs. CONCLUSIONS: Long-term exposure to a physiological shear stress in the retinal arterioles up-regulated eNOS and TM mRNA expressions and downregulated ET-1 mRNA expression in HRMECs. These results suggest that shear stress may be associated with the vasoregulatory and antithrombotic properties of retinal vessels under physiological conditions present during retinal circulation. |
| ISSN | 01460404 |
| e-ISSN | 15525783 |
| Journal | Investigative Opthalmology & Visual Science |
| Issue Number | 11 |
| Volume Number | 52 |
| Language | English |
| Publisher | Association for Research in Vision and Ophthalmology |
| Publisher Date | 2011-10-31 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Endothelin-1 Genetics Endothelium, Vascular Metabolism Gene Expression Regulation Physiology Nitric Oxide Synthase Type Iii Retinal Artery Stress, Mechanical Thrombomodulin Arterioles Cells, Cultured Rna, Messenger Real-time Polymerase Chain Reaction Time Factors Research Support, Non-u.s. Gov't Discipline Ophthalmology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ophthalmology Sensory Systems Cellular and Molecular Neuroscience |
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