NDLI: Biodiversity in the sorghum (Sorghum bicolor L. Moench) germplasm of Pakistan.

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Biodiversity in the sorghum (Sorghum bicolor L. Moench) germplasm of Pakistan.

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Biodiversity in the sorghum (Sorghum bicolor L. Moench) germplasm of Pakistan.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Iqbal, A. Awan, F. S. Khan, A. I. Sadaqat, H. A. Sadia, B. Kainth, R. A.
Spatial Coverage	Pakistan
Description	Country affiliation: Pakistan Author Affiliation: Iqbal A ( Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.)
Abstract	Sorghum ranks fifth in worldwide economic importance among cereal crops and is one of the most important summer annual grasses of Pakistan. As it is a very diverse crop, sorghum genetic fingerprinting requires an efficient marker system. We estimated genetic divergence among 29 sorghum (Sorghum bicolor) genotypes, including approved varieties and local and exotic lines collected from different ecological regions of Pakistan, using random amplified polymorphic DNA (RAPD) markers. A total of 125 RAPD loci, with an average of 66 loci per genotype, were used to calculate genetic divergence among these genotypes, of which 119 were polymorphic, showing 95% overall polymorphism. Genetic similarity ranged from 0.36 to 0.92, indicating a relatively broad genetic base. RAPD analysis revealed maximum similarity between the Indian III and K-A-113 sorghum genotypes (both exotic lines), while the F-601 and F-606 were observed to be the most diverse genotypes. Mean band frequency revealed by these RAPD primers ranged from 0.17 to 0.56, with an average of 0.36. The data presented here support the findings that RAPDs can be effectively used for studying genetic diversity in sorghum.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	2
Volume Number	9
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2010-04-27
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Biodiversity Genetic Variation Genetics Sorghum Cluster Analysis Dna Primers Metabolism Genotype Pakistan Phylogeny Quantitative Trait, Heritable Random Amplified Polymorphic Dna Technique Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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