NDLI: Molecular probes and microarrays for the detection of toxic algae in the genera Dinophysis and Phalacroma (Dinophyta).

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Reguera, Beatriz Groben, René Brubak, Sissel Rodríguez, Francisco Dittami, Simon M. Edvardsen, Bente Escalera, Laura Medlin, Linda K. Chen, Jixin
Spatial Coverage	Japan Spain New Zealand Norway Chile
Description	Author Affiliation: Edvardsen B ( Marine Biology, Department of Biology, University of Oslo, P.O. Box 1066, 0316, Oslo, Norway, bente.edvardsen@bio.uio.no.)
Abstract	Dinophysis and Phalacroma species containing diarrheic shellfish toxins and pectenotoxins occur in coastal temperate waters all year round and prevent the harvesting of mussels during several months each year in regions in Europe, Chile, Japan, and New Zealand. Toxicity varies among morphologically similar species, and a precise identification is needed for early warning systems. Molecular techniques using ribosomal DNA sequences offer a means to identify and detect precisely the potentially toxic species. We designed molecular probes targeting the 18S rDNA at the family and genus levels for Dinophysis and Phalacroma and at the species level for Dinophysis acuminata, Dinophysis acuta, and Dinophysis norvegica, the most commonly occurring, potentially toxic species of these genera in Western European waters. Dot blot hybridizations with polymerase chain reaction (PCR)-amplified rDNA from 17 microalgae were used to demonstrate probe specificity. The probes were modified along with other published fluorescence in situ hybridization and PCR probes and tested for a microarray platform within the MIDTAL project ( http://www.midtal.com ). The microarray was applied to field samples from Norway and Spain and compared to microscopic cell counts. These probes may be useful for early warning systems and monitoring and can also be used in population dynamic studies to distinguish species and life cycle stages, such as cysts, and their distribution in time and space.
ISSN	09441344
e-ISSN	16147499
DOI	10.1007/s11356-012-1403-1
Journal	Environmental Science and Pollution Research
Issue Number	10
Volume Number	20
Language	English
Publisher	Springer
Publisher Date	2013-10-01
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Dinoflagellida Genetics Environmental Monitoring Harmful Algal Bloom Oligonucleotide Array Sequence Analysis Phytoplankton Chile Dna, Ribosomal Metabolism Classification Growth & Development In Situ Hybridization, Fluorescence Marine Toxins Molecular Probes New Zealand Norway Polymerase Chain Reaction Shellfish Spain Research Support, Non-u.s. Gov't Discipline Environmental Science Discipline Environmental Chemistry
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine

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