| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Rittmann, Bruce E. Zhang, Yin Wen, Li-Lian Chen, Ran Tang, Youneng Chen, Jia-Xian Shi, Ling-Dong Luo, Yi-Hao Zheng, Ping Zhao, He-Ping |
| Description |
Country affiliation: China Author Affiliation: Chen R ( Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Zhejiang University, Hangzhou, Zhejiang, China.); Luo YH ( Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Zhejiang University, Hangzhou, Zhejiang, China.); Chen JX ( Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Zhejiang University, Hangzhou, Zhejiang, China.); Zhang Y ( Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Zhejiang University, Hangzhou, Zhejiang, China.); Wen LL ( Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Zhejiang University, Hangzhou, Zhejiang, China.); Shi LD ( MOE Key Laboratory of Environmental Remediation and Ecosystem Health, Zhejiang University, Hangzhou, China.); Tang Y ( Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou, China.); Rittmann BE ( Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou, China.); Zheng P ( Department of Civil and Environmental Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, 32310-6046, USA.); Zhao HP ( Swette Center for Environmental Biotechnology, Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, AZ, 85287-5701, USA.) |
| Abstract |
Previous work documented complete perchlorate reduction in a membrane biofilm reactor (MBfR) using methane as the sole electron donor and carbon source. This work explores how the biofilm’s microbial community evolved as the biofilm stage-wise reduced different combinations of perchlorate, nitrate, and nitrite. The initial inoculum, carrying out anaerobic methane oxidation coupled to denitrification (ANMO-D), was dominated by uncultured Anaerolineaceae and Ferruginibacter sp. The microbial community significantly changed after it was inoculated into the $CH_{4}-based$ MBfR and fed with a medium containing perchlorate and nitrite. Archaea were lost within the first 40 days, and the uncultured Anaerolineaceae and Ferruginibacter sp. also had significant losses. Replacing them were anoxic methanotrophs, especially Methylocystis, which accounted for more than 25 % of total bacteria. Once the methanotrophs became important, methanol-oxidizing denitrifying bacteria, namely, Methloversatilis and Methylophilus, became important in the biofilm, probably by utilizing organic matter generated by the metabolism of methanotrophs. When methane consumption was equal to the maximum-possible electron-donor supply, Methylomonas, also an anoxic methanotroph, accounted for >10 % of total bacteria and remained a major part of the community until the end of the experiments. We propose that aerobic methane oxidation coupled to denitrification and perchlorate reduction (AMO-D and AMO-PR) directly oxidized methane and reduced $NO_{3}^{−}$ to $NO_{2}^{−}$ or $N_{2}O$ under anoxic condition, producing organic matter for methanol-assimilating denitrification and perchlorate reduction (MA-D and MA-PR) to reduce $NO_{3}^{−}.$ Simultaneously, bacteria capable of anaerobic methane oxidation coupled to denitrification and perchlorate reduction (ANMO-D and ANMO-PR) used methane as the electron donor to respire $NO_{3}^{−}$ or $ClO_{4}^{−}$ directly. |
| ISSN |
09441344 |
| Issue Number |
10 |
| Journal |
Environmental Science and Pollution Research |
| Volume Number |
23 |
| e-ISSN |
16147499 |
| Language |
English |
| Publisher |
Springer |
| Publisher Date |
2016-05-01 |
| Publisher Place |
Germany |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Environmental Science Discipline Environmental Chemistry |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine |
