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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Su, Biling Chen, Guonan Tang, Dianping Tang, Juan |
| Description | Country affiliation: China Author Affiliation: Tang J ( Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education and Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, China.) |
| Abstract | A new electrochemical immunoassay protocol for sensitive detection of alpha-fetoprotein (AFP, as a model) is designed using carbon nanoparticles (CNPs)-functionalized biomimetic interface as immunosensing probe and irregular-shaped gold nanoparticles (ISNGs)-labeled horseradish peroxidase-anti-AFP conjugates (HRP-anti-AFP-ISNG) as trace label. The low-toxic and high-conductive CNPs provided a high capacity nanoparticulate immobilization surface and a facile pathway for electron transfer. In comparison with conventional label methods, i.e. spherical gold nanoparticles-labeled HRP-anti-AFP and HRP-labeled anti-AFP, the electrochemical immunosensor using HRP-anti-AFP-ISNGs as trace labels exhibited high bioelectrocatalytic response toward enzyme substrate and a wide dynamic range from 0.02 to 4.0 ng/mL with a low detection limit of 10 pg/mL toward AFP (at 3sigma). The developed immunoassay method showed good selectivity and acceptable reproducibility. Clinical serum samples with various AFP concentrations were evaluated by using the electrochemical immunosensor and the referenced enzyme-linked immunosorbent assay (ELISA), respectively, and received in good accordance with results obtained from these two methods. |
| ISSN | 09565663 |
| Issue Number | 12 |
| Volume Number | 25 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-08-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Immunoenzyme Techniques Metal Nanoparticles Nanotubes, Carbon Alpha-fetoproteins Analysis Antibodies, Immobilized Electric Conductivity Electrochemical Techniques Enzyme-linked Immunosorbent Assay Gold Horseradish Peroxidase Humans Limit Of Detection Ultrastructure Microscopy, Electron, Transmission Immunology Comparative Study Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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