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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yun, Minhee Huang, Jiyong Lee, Innam Hu, Yushi Luo, Xiliang Cui, Xinyan Tracy |
| Description | Country affiliation: United States Author Affiliation: Huang J ( Department of Electrical and Computer Engineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.) |
| Abstract | Single polypyrrole (PPy) nanowire-based microfluidic aptasensors were fabricated using a one-step electrochemical deposition method. The successful incorporation of the aptamers into the PPy nanowire was confirmed by fluorescence microscopy image. The microfluidic aptasensor showed responses to IgE protein solutions in the range from 0.01 nM to 100 nM, and demonstrated excellent specificity and sensitivity with faster response and rapid stabilization times (~20 s). At the lowest examined IgE concentration of 0.01 nM, the microfluidic aptasensor still exhibited ~0.32% change in the conductance. The functionality of this aptasensor was able to be regenerated using an acid treatment with no major change in sensitivity. In addition, the detection of cancer biomarker MUC1 was performed using another microfluidic aptasensor, which showed a very low detection limit of 2.66 nM MUC1 compared to commercially available MUC1 diagnosis assay (800 nM). |
| ISSN | 09565663 |
| e-ISSN | 18734235 |
| DOI | 10.1016/j.bios.2011.08.016 |
| Journal | Biosensors and Bioelectronics |
| Issue Number | 1 |
| Volume Number | 30 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-12-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Aptamers, Peptide Chemistry Biosensing Techniques Instrumentation Conductometry Microfluidic Analytical Techniques Nanotechnology Polymers Protein Array Analysis Pyrroles Computer Systems Electric Conductivity Equipment Design Equipment Failure Analysis Nanotubes Ultrastructure Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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