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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Prasad, Amrita Prasad, Bhim Bali Tiwari, Mahavir Prasad |
| Description | Country affiliation: India Author Affiliation: Prasad BB ( Analytical division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi-221005, India. prof.bbpd@yahoo.com) |
| Abstract | This study describes the synthesis of a new class of substrate-selective molecularly imprinted polymer. This involved tetraethylene glycol 3-morpholin propionate acrylate (functional monomer) and bovine serum albumin (template) for polymerization in aqueous condition, using 'surface grafting-from' approach directly on a vinyl exposed multiwalled carbon nanotubes-ceramic electrode. The analyte recapture at pH 6.8 in aqueous environment simultaneously involved hydrophobically driven hydrogen bonds and ionic interactions between negatively charged bovine serum albumin and positively charged imprinted nanofilm. The selectively encapsulated bovine serum albumin first gets reduced at -0.9V and then oxidized within the cavity, without getting stripped off, to respond a differential pulse voltammetry signal. The limit of detection [0.42ng mL(-1) (3σ, RSD≤1.02%)] obtained was free from any cross-reactivity and matrix complications in aqueous, pharmaceutical, serum, and liquid milk samples. The proposed sensor can be used as a practical sensor for ultra-trace analysis of bovine serum albumin in clinical settings. |
| ISSN | 09565663 |
| Issue Number | 1 |
| Volume Number | 39 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Ceramics Chemistry Electrochemical Techniques Instrumentation Molecular Imprinting Nanotubes, Carbon Polymers Serum Albumin, Bovine Analysis Animals Cattle Electrodes Humans Limit Of Detection Milk Pharmaceutical Preparations Surface Properties Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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