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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jeong, Heon-Ho Lee, Chang-Soo Lee, Sang-Ho |
| Description | Author Affiliation: Jeong HH ( Department of Chemical Engineering, Chungnam National University, Yuseong-Gu, Deajeon 305-764, Republic of Korea.) |
| Abstract | Bacterial chemotaxis is a complex response to temporal changes of concentrations of chemoeffectors. Conventional methods, including microfluidic approaches, for the precise analysis of bacterial chemotaxis are limited in the accurate control of chemical gradients, low sensitivity, and longer analytical times although the advances in microfluidic technology provide a novel platform. Here, we present a diffusion-based microfluidic device to provide rapid diffusion of chemoeffectors using a liquid-liquid interface, which is a critical advance in the analysis of bacterial chemotaxis. The microfluidic method can rapidly analyze attractants and repellents, achieving a chemotaxis index corresponding to the concentrations and types of chemoeffectors. Moreover, we find that the dynamic switch in bacterial chemotaxis from attraction to repellence in response to specific chemoeffectors occurs in a concentration-dependent manner. Thus, our microfluidic method is promising and reliable for the analysis of bacterial chemotaxis. |
| ISSN | 09565663 |
| Volume Number | 47 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Chemotaxis Microfluidic Analytical Techniques Instrumentation Pseudomonas Aeruginosa Isolation & Purification Capillary Action Diffusion Humans Pathogenicity Wetting Agents Chemistry Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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