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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hu, Xinjiang Fu, Xin Zhang, He |
| Description | Author Affiliation: Zhang H ( School of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, People׳s Republic of China. Electronic address: mzhang_he@126.com.); Hu X ( School of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, People׳s Republic of China.); Fu X ( School of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, People׳s Republic of China.) |
| Abstract | This study reports the development of an aptamer-mediated microfluidic beads-based sensor for multiple analytes detection and quantification using multienzyme-linked nanoparticle amplification and quantum dots labels. Adenosine and cocaine were selected as the model analytes to validate the assay design based on strand displacement induced by target-aptamer complex. Microbeads functionalized with the aptamers and modified electron rich proteins were arrayed within a microfluidic channel and were connected with the horseradish peroxidases (HRP) and capture DNA probe derivative gold nanoparticles (AuNPs) via hybridization. The conformational transition of aptamer induced by target-aptamer complex contributes to the displacement of functionalized AuNPs and decreases the fluorescence signal of microbeads. In this approach, increased binding events of HRP on each nanosphere and enhanced mass transport capability inherent from microfluidics are integrated for enhancing the detection sensitivity of analytes. Based on the dual signal amplification strategy, the developed aptamer-based microfluidic bead array sensor could discriminate as low as 0.1 pM of adenosine and 0.5 pM cocaine, and showed a 500-fold increase in detection limit of adenosine compared to the off-chip test. The results proved the microfluidic-based method was a rapid and efficient system for aptamer-based targets assays (adenosine (0.1 pM) and cocaine (0.5 pM)), requiring only minimal (microliter) reagent use. This work demonstrated the successful application of aptamer-based microfluidic beads array sensor for detection of important molecules in biomedical fields. |
| ISSN | 09565663 |
| Volume Number | 57 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-07-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Adenosine Analysis Aptamers, Nucleotide Chemistry Biosensing Techniques Instrumentation Cocaine Microfluidic Analytical Techniques Quantum Dots Blood Equipment Design Gold Horseradish Peroxidase Humans Limit Of Detection Microspheres Nanoparticles Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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