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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ibrahim, Mouhamad Senez, Vincent Dibao-Dina, Alfred Follet, Jérôme Vlandas, Alexis |
| Description | Country affiliation: France Author Affiliation: Dibao-Dina A ( Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN), CNRS, UMR 8520, 59652 Villeneuve d'Ascq, France.); Follet J ( Laboratoire de Biotechnologies des Agents Pathogènes en Agriculture, Etablissement ISA groupe HEI-ISA-ISEN, 59046 Lille, France. Electronic address: jerome.follet@isa-lille.fr.); Ibrahim M ( Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN), CNRS, UMR 8520, 59652 Villeneuve d'Ascq, France.); Vlandas A ( Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN), CNRS, UMR 8520, 59652 Villeneuve d'Ascq, France. Electronic address: alexis.vlandas@iemn.univ-lille1.fr.); Senez V ( Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN), CNRS, UMR 8520, 59652 Villeneuve d'Ascq, France.) |
| Abstract | Cryptosporidium is the main origin of worldwide waterborne epidemic outbreaks caused by protozoan parasites. Its resilience to water chemical treatments and the absence of therapy led to consider it as a reference pathogen to assess water quality and as a possible bioterrorism agent. We here show that an electrical impedance-based device is able to get insights on Cryptosporidium development on a cell culture and to quantify sample infectivity. HCT-8 cells were grown to confluency on Interdigitated Microelectrode Arrays (IMA's) during 76h and then infected by Cryptosporidium parvum during 60h. The impedimetric response was measured at frequencies ranging from 100Hz to 1MHz and a 7min sampling period. As the infection progresses the impedance signal shows a reproducible distinct succession of peaks at 12h post infection (PI), 23h PI and 31h PI and local minima at 9h PI, 19h PI and 28h PI. An equivalent circuit modeling-based approach indicates that these features are mostly originated from paracellular pathway modifications due to host-parasite interactions. Furthermore, our data present for the first time a real-time monitoring of early parasitic stage development with alternating zoite and meront predominances, observed respectively at peaks and local minima in the impedimetric signal. Finally, by quantifying the magnitude of the impedimetric response, we demonstrate this device can also be used as an infectivity sensor as early as 12h PI thus being at least 6 times faster than other state of the art techniques. |
| ISSN | 09565663 |
| Volume Number | 66 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Cryptosporidiosis Physiopathology Cryptosporidium Parvum Physiology Host-parasite Interactions Intestines Cytology Parasitology Cell Line, Tumor Electric Impedance Equipment Design Humans Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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